Assessment of tumoral PD-L1 expression and intratumoral CD8+ T cells in urothelial carcinoma.

Abstract

OBJECTIVE:

To evaluate programmed death ligand 1 (PD-L1) expression in urothelial carcinoma of the bladder in relationship with tumor-infiltrating CD8+ T cells.

MATERIALS AND METHODS:

Tissue microarrays were prepared from 56 cystectomy specimens performed at our hospital (1994-2002). PD-L1 immunoexpression was assessed using the murine antihuman PD-L1 monoclonal antibody 5H1. Extent of membranous PD-L1 expression was assigned in each spot. Spots showing ≥5% expression were considered positive. Average PD-L1 expression per tumor was also calculated (5% positivity cutoff). "High CD8 density" was defined as the presence of ≥60 CD8+ intraepithelial lymphocytes per high power field in a given spot. A tumor was considered high density if ≥50% of its spots were of high density.

RESULTS:

PD-L1 expression was positive in approximately 20% of tumors. None of the benign urothelium spots expressed PD-L1. High CD8 density was observed in approximately 20% of cases. CD8 density did not correlate with PD-L1 expression. Overall survival (OS) and disease-specific survival (DSS) rates were 14% and 28%, respectively (median follow-up, 31.5 months). PD-L1 expression was associated with age at cystectomy (P = .01). Remaining clinicopathologic parameters were not associated with PD-L1 expression or CD8 density. High CD8 density was associated with favorable OS (P = .02) and DSS (P = .02). The same was true when CD8 density was adjusted for demographic and clinicopathologic parameters. There was no correlation between PD-L1 expression and outcome.

CONCLUSION:

High intratumoral CD8+ T cell density is associated with better OS and DSS in invasive urothelial carcinoma of the bladder. We found no correlation between PD-L1 expression and outcome.

• Authors: Sheila F Faraj, Enrico Munari, Gunes Guner, Janis Taube, Robert Anders, Jessica Hicks, Alan Meeker, Mark Schoenberg, Trinity Bivalacqua, Charles Drake, George J Netto
• Journal: Urology (Urology) Vol. 85 Issue 3 Pg. 703.e1-6 (Mar 2015) ISSN: 1527-9995 [Electronic] United States
• PMID: 25733301 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• B7-H1 Antigen

Topics

• Adult
• Aged
• Aged, 80 and over
• B7-H1 Antigen (biosynthesis)
• CD8-Positive T-Lymphocytes
• Carcinoma, Transitional Cell (immunology, metabolism, pathology)
• Female
• Humans
• Lymphocytes, Tumor-Infiltrating
• Male
• Middle Aged
• Retrospective Studies
• Urinary Bladder Neoplasms (immunology, metabolism)