Abstract | BACKGROUND AND AIMS: MATERIALS AND METHODS: MTT assay was performed to assess the proliferation of Hela, MCF-7 and NB4 cells. Isobolographic analysis was used to evaluate combination index values from cell viability data. The apoptosis and the cellular reactive oxygen species (ROS) level were assessed by fluorescent microscopy and flow cytometry separately in vitro. The effect of As2O3, alone and in combination with resveratrol on Hela tumor growth in an orthotopic nude mouse model was also investigated. The tumor volume and the immunohistochemical analysis of CD31, CD34 and VEGF were determined. RESULTS:
Resveratrol dramatically enhanced the anti- cancer effect induced by As2O3 in vitro. In addition, isobolographic analysis further demonstrated that As2O3 and resveratrol generated a synergistic action. More apoptosis and ROS generation were observed in the combination treatment group. Similar synergistic effects were found in nude mice in vivo. The combination of As2O3 and resveratrol dramatically suppressed both tumor growth and angiogenesis in nude mice. CONCLUSIONS:
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Authors | Xiao-Yan Zhao, Shen Yang, You-Ran Chen, Pei-Chun Li, Meng-Meng Dou, Jie Zhang |
Journal | PloS one
(PLoS One)
Vol. 9
Issue 6
Pg. e98925
( 2014)
ISSN: 1932-6203 [Electronic] United States |
PMID | 24901647
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Arsenicals
- Oxides
- Reactive Oxygen Species
- Stilbenes
- Resveratrol
- Arsenic Trioxide
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Topics |
- Animals
- Antineoplastic Agents
(administration & dosage, pharmacology)
- Apoptosis
(drug effects)
- Arsenic Trioxide
- Arsenicals
(administration & dosage, pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Disease Models, Animal
- Drug Synergism
- Humans
- Male
- Mice
- Necrosis
(drug therapy, pathology)
- Neovascularization, Pathologic
(drug therapy, metabolism, pathology)
- Oxides
(administration & dosage, pharmacology)
- Reactive Oxygen Species
(metabolism)
- Resveratrol
- Stilbenes
(administration & dosage, pharmacology)
- Tumor Burden
(drug effects)
- Xenograft Model Antitumor Assays
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