Effects of recombinant granulocyte colony-stimulating factor on Staphylococcus aureus mastitis in lactating dairy cows.

Abstract

The in vivo effects of recombinant human granulocyte colony-stimulating factor on blood and milk leukocytes in dairy cows was examined. A 2-fold increase in peripheral white blood cell counts was observed by d 5 of treatment and peaked on d 12 with values 3-fold those of controls. Counts remained elevated above pretreatment values during the treatment period, then returned to normal by d 23 of the trial. Differential white blood cell counts demonstrated that neutrophils predominated (73.8%) in treated cows versus controls (22.1%) during the treatment period. Immediately prior to experimental challenge with Staphylococcus aureus, milk SCC were 582 x 10(3) and 261 x 10(3)/ml, and percentages milk neutrophils were 64.4 and 45.3, respectively, in treated and control cows. After challenge, a 46.7% reduction in new infections was observed in quarters of treated cows compared with controls. Recombinant human granulocyte colony-stimulating factor was a granulopoietic growth and differentiation factor in the cow, and the resulting leukocytosis into the mammary gland may have been protective against experimental bacterial challenge.

• Authors: S C Nickerson, W E Owens, J L Watts
• Journal: Journal of dairy science (J Dairy Sci) Vol. 72 Issue 12 Pg. 3286-94 (Dec 1989) ISSN: 0022-0302 [Print] United States
• PMID: 2483406 (Publication Type: Journal Article)

Chemical References

• Colony-Stimulating Factors
• Recombinant Proteins
• Granulocyte Colony-Stimulating Factor

Topics

• Animals
• Cattle
• Cell Count (veterinary)
• Colony-Stimulating Factors (therapeutic use)
• Female
• Granulocyte Colony-Stimulating Factor
• Lactation
• Leukocyte Count (veterinary)
• Leukocytes (immunology)
• Mastitis, Bovine (prevention & control)
• Milk (cytology)
• Pregnancy
• Recombinant Proteins (therapeutic use)
• Staphylococcal Infections (prevention & control, veterinary)