Abstract | OBJECTIVE: METHODS: MDA-MB-231 cells were treated by PARP inhibitor AG014699 alone or combination with DTX or CBP. Cell proliferation was measured by cell counting kit-8 assay. The combined effect was evaluated by q value less than 0.85, in the range of 0.85 and 1.15, more than 1.15, which respectively meant that the combined effect of the drugs was antagonistic, additive, and synergistic. RESULTS: Treatment with PARP inhibitor AG014699, DTX, or CBP alone inhibited the proliferation, induced apoptosis and blocked the cell cycle. The cell viability of AG014699 (10 µmol/L) combined with DTX (10(-8), 10(-7), 10(-6), 10(-5) mol/L) or CBP (10(-5), 10(-4) mol/L) were lower than that of the drug used alone, and q value was between 0.85 and 1.15, suggesting the combined effect was additive. The cell viability of AG014699 (10 µmol/L) combined with CBP (10(-3) mol/L) was lower than that of the drug used alone, and q value was more than 1.15, suggesting the combined effect was synergetic. A combination of PARP inhibitor AG014699 and DTX or CBP promoted apoptosis and increased the proportion of G2/M stage cells. CONCLUSION:
PARP inhibitor AG014699 combined with DTX or CBP can remarkably inhibit MDA-MB-231 cell proliferation, showing additive or synergistic antitumor effects.
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Authors | Ying Sun, Huan Ding, Xiao-qing Li, Li Li |
Journal | Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae
(Zhongguo Yi Xue Ke Xue Yuan Xue Bao)
Vol. 36
Issue 2
Pg. 135-9
(Apr 2014)
ISSN: 1000-503X [Print] China |
PMID | 24791791
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Enzyme Inhibitors
- Indoles
- rucaparib
- Carboplatin
|
Topics |
- Antineoplastic Combined Chemotherapy Protocols
- Apoptosis
(drug effects)
- Carboplatin
(pharmacology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Enzyme Inhibitors
(pharmacology)
- Female
- Humans
- Indoles
(pharmacology)
- Triple Negative Breast Neoplasms
(pathology)
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