ABCG2 gene amplification and expression in esophageal cancer cells with acquired adriamycin resistance.

Abstract

Resistance to chemotherapeutic agents is the main reason for treatment failure in patients with cancer. The primary mechanism of multidrug resistance (MDR) is the overexpression of drug efflux transporters, including ATP‑binding cassette transporter G2 (ABCG2). To the best of our knowledge, the MDR mechanisms of esophageal cancer have not been described. An adriamycin (ADM)-resistant subline, Eca109/ADM, was generated from the Eca109 esophageal cancer cell line by a stepwise selection in ADM from 0.002 to 0.02 ng/µl. The resulting subline, designated Eca109/ADM, revealed a 3.29-fold resistance against ADM compared with the Eca109 cell line. The ABCG2 gene expression in the Eca109/ADM cells was increased compared with that of the Eca109 cells. The cellular properties of the Eca109/ADM cells were detected by reverse transcription polymerase chain reaction (RT-PCR), flow cytometry and western blotting. The ABCG2 expression levels were detected by RT-PCR and flow cytometry, and the drug efflux effect was detected by flow cytometry. The present study detected the correlation between ABCG2 and the multidrug resistance of esophageal cancer. ABCG2 gene expression and the drug efflux effect of the Eca109/ADM cells were increased compared with those of the Eca109 cells. Collectively, the results of this study indicated that the overexpression of ABCG2 in the Eca109/ADM cells resulted in drug efflux, which may be responsible for the development of esophageal cancer MDR.

• Authors: Liang Liu, Lian Fu Zuo, Jian Wen Guo
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 9 Issue 4 Pg. 1299-304 (Apr 2014) ISSN: 1791-3004 [Electronic] Greece
• PMID: 24535197 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ABCG2 protein, human
• ATP Binding Cassette Transporter, Subfamily G, Member 2
• ATP-Binding Cassette Transporters
• Neoplasm Proteins
• RNA, Messenger
• Doxorubicin

Topics

• ATP Binding Cassette Transporter, Subfamily G, Member 2
• ATP-Binding Cassette Transporters (genetics, metabolism)
• Apoptosis (drug effects, genetics)
• Blotting, Western
• Cell Line, Tumor
• Cell Shape (drug effects, genetics)
• Doxorubicin (pharmacology)
• Drug Resistance, Neoplasm (drug effects, genetics)
• Esophageal Neoplasms (genetics, pathology)
• Fluorescence
• Gene Amplification (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Neoplasm Proteins (genetics, metabolism)
• Phenotype
• RNA, Messenger (genetics, metabolism)