Preparation and evaluation of polysaccharide sulfates for inhibiting Helicobacter pylori adhesion.

Abstract

In treatments of Helicobacter pylori infections, recrudescences were common because of an unfavorable bacterial eradication rate due to the ever increasing resistance to antibiotics. In this study, we chose pectin, guar gum and chitosan to synthesize their sulfates to inhibit adhesions of H. pylori and thus enhance the eradication rate. The introduction of sulfates was characterized using FT-IR and elemental analysis. Data from zeta-potential, hydrodynamic diameter, hydrolysis and rheological property demonstrated the sulfates were physicochemically stable. Inhibition assay of hemagglutination and adhesion indicated sulfates prevented H. pylori from adhering to erythrocytes and AGS cells. In binding assay, affinities of sulfates to H. pylori suggested sulfates could compete with target cells for bacteria and moderated the bacterial adhesion to hosts. A higher content of galactoses and 2,3-O-linked sulfates benefited this action. Thus polysaccharide sulfates can serve as potential adjuvants to raise the bacterial eradication rate by inhibiting adhesions of H. pylori.

• Authors: Weijuan Song, Yalong Wang, Liyan Zhang, Shengnan Fu, Ying Zeng, Haiyan Hu
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 103 Pg. 398-404 (Mar 15 2014) ISSN: 1879-1344 [Electronic] England
• PMID: 24528746 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2013 Elsevier Ltd. All rights reserved.

Chemical References

• Adhesins, Bacterial
• Polysaccharides
• Sulfates

Topics

• Adhesins, Bacterial (drug effects)
• Dose-Response Relationship, Drug
• Helicobacter pylori (drug effects)
• Polysaccharides (chemical synthesis, chemistry, pharmacology)
• Structure-Activity Relationship
• Sulfates (chemical synthesis, chemistry, pharmacology)