Abstract |
The aim of this study is to identify a plasticizer that is effective in the suppression of the autohemolysis of the stored blood and can be used to replace di(2-ethylhexyl) phthalate ( DEHP) in blood containers. The results of hemolysis test using mannitol- adenine- phosphate/red cell concentrates (MAP/RCC) spiked with plasticizers included phthalate, phthalate-like, trimeliate, citrate, and adipate derivatives revealed that di-isononyl-cyclohexane-1,2-dicarboxylate ( Hexamoll(®) DINCH), di(2-ethylhexyl)-1,2,3,6-tetrahydro-phthalate ( DOTP), and diisodecyl phthalate (DIDP) exhibited a hemolysis suppression effect almost equal to that of DEHP, but not other plasticizers. This finding suggested that the presence of 2 carboxy- ester groups at the ortho position on a 6-membered ring of carbon atoms may be required to exhibit such an effect. The hemolytic ratios of MAP/RCC-soaked polyvinyl chloride ( PVC) sheets containing DEHP or different amounts of DINCH or DOTP were reduced to 10.9%, 9.2-12.4%, and 5.2-7.8%, respectively (MAP/RCC alone, 28.2%) after 10 weeks of incubation. The amount of plasticizer eluted from the PVC sheet was 53.1, 26.1-36.5, and 78.4-150 µg/mL for DEHP, DINCH, and DOTP, respectively. PVC sheets spiked with DIDP did not suppress the hemolysis induced by MAP/RCC because of low leachability (4.8-6.0 µg/mL). These results suggested that a specific structure of the plasticizer and the concentrations of least more than ∼10 µg/mL were required to suppress hemolysis due to MAP/RCC.
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Authors | Yuji Haishima, Tsuyoshi Kawakami, Chie Hasegawa, Akito Tanoue, Toshiyasu Yuba, Kazuo Isama, Atsuko Matsuoka, Shingo Niimi |
Journal | Journal of biomedical materials research. Part B, Applied biomaterials
(J Biomed Mater Res B Appl Biomater)
Vol. 102
Issue 4
Pg. 721-8
(May 2014)
ISSN: 1552-4981 [Electronic] United States |
PMID | 24155207
(Publication Type: Comparative Study, Journal Article)
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Copyright | Copyright © 2013 Wiley Periodicals, Inc. |
Chemical References |
- Benzoates
- Citrates
- Cyclohexanecarboxylic Acids
- Dicarboxylic Acids
- Inosine Nucleotides
- Oxazoles
- Plasticizers
- Pyrimidinones
- deoxyinosine diphosphate
- diisononyl 1,2-cyclohexanedicarboxylic acid
- 2,3-dihydro-5H-oxazolo(3,2-a)thieno(3,2-d)pyrimidin-5-one
- Mannitol
- citrate phosphate dextrose
- Polyvinyl Chloride
- Heparin
- trioctyl trimellitate
- Diethylhexyl Phthalate
- Glucose
- Adenine
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Topics |
- Adenine
- Benzoates
(pharmacology)
- Blood Preservation
(instrumentation)
- Citrates
- Cyclohexanecarboxylic Acids
(pharmacology)
- Depression, Chemical
- Dicarboxylic Acids
(pharmacology)
- Diethylhexyl Phthalate
(pharmacology, toxicity)
- Gas Chromatography-Mass Spectrometry
- Glucose
- Hemolysis
(drug effects)
- Heparin
- Humans
- Inosine Nucleotides
(pharmacology)
- Mannitol
- Oxazoles
(pharmacology)
- Plasticizers
(chemistry, pharmacology)
- Polyvinyl Chloride
- Pyrimidinones
(pharmacology)
- Structure-Activity Relationship
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