Abstract |
A triple helical polysaccharide (PD3) was isolated from Dictyophora indusiata. After denaturation in dilute NaOH solution (0.3 M) and renaturation by sequential dialysis, regenerated polysaccharide (RPD3) was obtained. The physico-chemical properties of RPD3 including intrinsic viscosity [η], molecular weight (Mw) and optical rotation were similar to those of PD3, which suggested that RPD3 also had a triple helical structure after denaturation-renaturation. However, different intrinsic viscosity dependence on the concentration of NaOH solution was noted in PD3 and RPD3, which indicated that RPD3 had lower chain tightness compared with PD3. The anti- tumor activity of this polysaccharide after denaturation-renaturation treatment was further investigated. Both PD3 and RPD3 showed no direct cytotoxicity against S-180 cells in vitro but behaved anti- tumor activity in vivo. Meanwhile, RPD3 in high-dose group showed much higher anti- tumor activity than that of PD3, suggesting that the denaturation-renaturation treatment improved the bioactivity of the polysaccharide from D. indusiata.
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Authors | Chao Deng, Haitian Fu, Liping Teng, Zhun Hu, Xiaofei Xu, Jinghua Chen, Tianli Ren |
Journal | International journal of biological macromolecules
(Int J Biol Macromol)
Vol. 61
Pg. 453-8
(Oct 2013)
ISSN: 1879-0003 [Electronic] Netherlands |
PMID | 23973497
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2013 Elsevier B.V. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Cytokines
- Fungal Polysaccharides
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Topics |
- Animals
- Antineoplastic Agents
(chemistry, pharmacology, toxicity)
- Basidiomycota
(chemistry)
- Body Weight
(drug effects)
- Cell Line, Tumor
- Cytokines
(blood, metabolism)
- Fungal Polysaccharides
(chemistry, pharmacology, toxicity)
- Male
- Mice
- Molecular Weight
- Neoplasms
(immunology, metabolism, pathology)
- Viscosity
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