[Effect of Fasudil on miroglia and astrocytes in experimental autoimmune encephalomyelitis mice].

Abstract

AIM:

To explore the therapeutic effect of Fasudil and its possible mechanisms in experimental autoimmune encephalomyelitis (EAE) mice, mainly focusing on the roles of microglia and astrocytes in the treatment.

METHODS:

Female adult C57BL/6 mice were immunized with MOG35-55 to induce chronic EAE. Fasudil was injected on day 3 p.i. (early Fasudil treatment), or at the onset of EAE (late Fasudil treatment). Normal saline was injected in other mice as EAE controls in a similar manner. Clinical score and body mass were recorded every other day. The expressions of iNOS on microglia and p-NF-κB/p65 on astrocytes were measured by immunohistochemistry and Western blotting. The levels of IL-1β and TNF-α in spinal cord homogenate were determined by ELISA.

RESULTS:

Fasudil delayed onset and ameliorated the severity of EAE. Fasudil inhibited the expression of iNOS on microglia and p-NF-κB/p65 on astrocytes in spinal cords, accompanied by the inhibition of inflammatory factors IL-1β and TNF-α.

CONCLUSION:

Fasudil exhibits therapeutic effect on EAE, possibly through inhibiting inflammatory molecules on microglia and astrocyte.

• Authors: Yan-hua Li, Chun-yun Liu, Pei-jun Zhang, Jie-zhong Yu, Ning Ji, Yan-yan Yan, Ling Feng, Hai-fei Zhang, Bao-guo Xiao, Cun-gen Ma
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 28 Issue 12 Pg. 1242-5 (Dec 2012) ISSN: 1007-8738 [Print] China
• PMID: 23232512 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1beta
• NF-kappa B
• Tumor Necrosis Factor-alpha
• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• fasudil

Topics

• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine (administration & dosage, analogs & derivatives)
• Animals
• Astrocytes (drug effects, immunology)
• Encephalomyelitis, Autoimmune, Experimental (drug therapy, genetics, immunology)
• Female
• Humans
• Interleukin-1beta (genetics, immunology)
• Mice
• Mice, Inbred C57BL
• NF-kappa B (genetics, immunology)
• Neuroglia (drug effects, immunology)
• Spinal Cord (drug effects, immunology)
• Tumor Necrosis Factor-alpha (genetics, immunology)