BRCA1 germ-line mutations and tumor characteristics in eastern Chinese women with familial breast cancer.

Abstract

Although several studies detected the BRCA1 germ-line mutations in Chinese women with familial breast cancer, most of them did not employ conventional full gene sequencing, especially in eastern China. In addition, the clinicopathological features of BRCA1-associated breast cancer in Chinese women were not well investigated. In this study, we screened the complete coding regions and exon-intron boundaries of BRCA1 by polymerase chain reaction (PCR)-sequencing assay. Immunohistochemistry analyses were performed on tumor samples to detect the expression of estrogen receptor (ER), progesterone receptor (PR), P53, and human epidermal growth factor receptor-2 (HER-2). Breast cancer patients having one or more affected relatives referred from the Zhejiang Cancer Hospital, eastern China during 2008-2011 were selected for the study. A total of 62 familial breast cancer patients received the BRCA1 germ-line mutation screening. Five deleterious mutations were detected in this cohort. The mutation rate was 11.3% (7/62). We found two novel mutations (3414delC and 5,280 C > T) and two recurrent mutations (5,273 G > A and 5589del8). BRCA1 mutation tumors tended to be negative for ER, PR, and HER-2, and exhibited high histological grade compared with tumors without BRCA1 mutations. Our study suggests that recurrent mutations may exist in eastern Chinese women with familial breast cancer and PCR-sequencing assay is a useful tool to screen these mutations. It also suggests that BRCA1-associated breast cancers in Chinese women exhibit an aggressive phenotype.

• Authors: Wenming Cao, Xiaojia Wang, Yun Gao, Hongjian Yang, Ji-Cheng Li
• Journal: Anatomical record (Hoboken, N.J. : 2007) (Anat Rec (Hoboken)) Vol. 296 Issue 2 Pg. 273-8 (Feb 2013) ISSN: 1932-8494 [Electronic] United States
• PMID: 23175448 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Wiley Periodicals, Inc.

Chemical References

• BRCA1 Protein
• BRCA1 protein, human
• Biomarkers, Tumor

Topics

• Adult
• Aged
• Asian People (genetics)
• BRCA1 Protein (genetics)
• Biomarkers, Tumor (analysis)
• Breast Neoplasms (chemistry, ethnology, genetics, pathology)
• Chi-Square Distribution
• China (epidemiology)
• DNA Mutational Analysis (methods)
• Female
• Genetic Predisposition to Disease
• Germ-Line Mutation
• Humans
• Immunohistochemistry
• Middle Aged
• Mutation Rate
• Neoplasm Grading
• Pedigree
• Phenotype
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Young Adult