Streptococcus pneumoniae exposure is associated with human metapneumovirus seroconversion and increased susceptibility to in vitro HMPV infection.

Abstract

It remains largely unknown which factors determine the clinical outcome of human metapneumovirus (HMPV) infections. The aim of the present study was to analyse whether exposure to bacterial pathogens can influence HMPV infections. From 57 children, serum samples and colonization data for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Streptococcus pneumoniae were collected at 1.5, 6, 14 and 24 months of age. Seroconversion rates to HMPV were determined and related to bacterial carriage. Frequent nasopharyngeal carriage (≥2 times in the first 2 years of life) of S. pneumoniae, but not of the other three pathogens, was associated with increased seroconversion rates of infants to HMPV at the age of 2 years (frequently vs. less exposed, 93% vs. 59%; p <0.05). Subsequently, the susceptibility of well-differentiated normal human bronchial epithelial cells (wd-NHBE) pre-incubated with bacterial pathogens to in vitro HMPV infection was evaluated. Pre-incubation of wd-NHBE with S. pneumoniae resulted in increased susceptibility to infection with HMPV-enhanced green fluorescent protein (EGFP), as determined by enumeration of EGFP-positive cells. This was not the case for cells pre-incubated with H. influenzae, M. catarrhalis on S. aureus. We conclude that exposure to S. pneumoniae can modulate HMPV infection.

• Authors: N J Verkaik, D T Nguyen, C P de Vogel, H A Moll, H A Verbrugh, V W V Jaddoe, A Hofman, W J B van Wamel, B G van den Hoogen, R M G B Buijs-Offerman, M Ludlow, L de Witte, A D M E Osterhaus, A van Belkum, R L de Swart
• Journal: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases (Clin Microbiol Infect) Vol. 17 Issue 12 Pg. 1840-4 (Dec 2011) ISSN: 1469-0691 [Electronic] England
• PMID: 21883660 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.

Chemical References

• Antibodies, Viral

Topics

• Antibodies, Viral (blood)
• Carrier State
• Child, Preschool
• Disease Susceptibility
• Haemophilus influenzae (isolation & purification, pathogenicity)
• Humans
• Infant
• Metapneumovirus (immunology, isolation & purification)
• Moraxella catarrhalis (isolation & purification, pathogenicity)
• Nasopharynx (microbiology)
• Paramyxoviridae Infections (epidemiology, immunology, virology)
• Pneumococcal Infections (complications)
• Staphylococcus aureus (isolation & purification, pathogenicity)
• Streptococcus pneumoniae (isolation & purification, pathogenicity)