[Deficiency of water channel AQP4 aggravates NMDA-induced brain injury in mice].

Abstract

OBJECTIVE:

To evaluate the role of water channel AQP4 in NMDA-induced brain injury in mice.

METHODS:

In AQP4 gene knockout (AQP4(-/-)) mice, brain injury was induced by microinjection of NMDA into the cortex. The injured area was determined by toluidine blue staining, degenerated neurons were detected by Fluro-Jade B staining, and increased blood-brain barrier (BBB) permeability was evaluated by IgG immunostaining.

RESULT:

Compared with wild-type mice, AQP4(-/-) mice exhibited increased cortical lesion area, aggravated neuron degeneration, and increased BBB disruption after NMDA microinjection.

CONCLUSION:

AQP4 may play a protective role in NMDA-induced brain injury in mice.

• Authors: Wen-Zhen Shi, Chun-Zhen Zhao, Xue-Qin Huang, Wei-Ping Zhang, San-Hua Fang, Yun-Bi Lu, Er-Qing Wei
• Journal: Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences (Zhejiang Da Xue Xue Bao Yi Xue Ban) Vol. 40 Issue 2 Pg. 145-9 (03 2011) ISSN: 1008-9292 [Print] China
• PMID: 21488209 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aqp4 protein, mouse
• Aquaporin 4
• N-Methylaspartate

Topics

• Animals
• Aquaporin 4 (genetics, physiology)
• Blood-Brain Barrier (pathology)
• Brain (drug effects, pathology)
• Mice
• Mice, Knockout
• N-Methylaspartate (toxicity)