Resveratrol as an adjunct therapy in cyclophosphamide-treated MCF-7 cells and breast tumor explants.

Abstract

Cyclophosphamide (CPA) has efficacy as a breast cancer therapy. However, toxicity to CPA limits its clinical applications. Hence there is a need to develop compounds that may be combined with it to improve the efficacy and overcome toxicity. We showed previously that Resveratrol (RES), a chemopreventive agent, increased the growth inhibitory effect of CPA-treated MCF-7 cells. Here we have explored the molecular basis of 5 mM CPA and 50 μM RES as a combination on cell-cycle progression, apoptosis and oxidative stress in MCF-7 breast cancer cells. Efficacy of the combination was also evaluated in a serum-free tumor explant culture model. The combination elicited enhanced anti-proliferative action coupled with differential expression of cell-cycle, apoptosis and stress factors. Furthermore, co-treatment superiority in histologically validated ER positive breast cancer explants suggests that this combination may be a worthy future clinical anti-neoplastic regimen.

• Authors: Neetu Singh, Manisha Nigam, Vishal Ranjan, Deeba Zaidi, Vivek Kumar Garg, Sharad Sharma, Rashmi Chaturvedi, Rishi Shankar, Sadan Kumar, Ramesh Sharma, Kalyan Mitra, Anil K Balapure, Srikanta K Rath
• Journal: Cancer science (Cancer Sci) Vol. 102 Issue 5 Pg. 1059-67 (May 2011) ISSN: 1349-7006 [Electronic] England
• PMID: 21276137 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 Japanese Cancer Association.

Chemical References

• Stilbenes
• Cyclophosphamide
• Resveratrol

Topics

• Antineoplastic Combined Chemotherapy Protocols (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Breast Neoplasms (drug therapy, pathology, ultrastructure)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Chemotherapy, Adjuvant
• Cyclophosphamide (administration & dosage)
• Female
• Humans
• Immunoprecipitation
• Microscopy, Electron, Transmission
• Middle Aged
• Oxidative Stress (drug effects)
• Resveratrol
• Stilbenes (administration & dosage)
• Tumor Cells, Cultured