The clinical implications of MMP-11 and CK-20 expression in human breast cancer.

Abstract

BACKGROUND:

Tumor invasiveness and metastasis in cancer progression is manifested by epigenetic abnormality. However, it remains unknown whether transcription regulation of matrix metalloproteinase-11(MMP-11) and cytoskeleton-20 (CK-20) genes for the homoeostasis of epithelial/connective interface that can enhance cell dissemination and invasion may act as alternative mutators to tumor clinicopathology.

METHODS:

Paired cancerous and tumor-adjacent normal tissues from 72 breast cancer patients were assayed for the expression of MMP-11 and CK-20 by using real-time RT-PCR. The expression profiles were evaluated for the association with clinicopathological factors.

RESULTS:

Breast tumor tissues displayed higher expression levels of MMP-11 and CK-20 than those of the adjacent non-cancerous tissues. Overexpression of either MMP-11 or CK-20 correlated with patients having poorly differentiated tumors (P(MMP-11)=0.01 and P(CK-20)=0.05) and lymph node metastasis (LNM) (P(MMP-11)=0.004 and P(CK-20)=0.001). A synergistic effect between MMP-11 and CK-20 on risk elevation was significant in patients with advanced tumor stage (OR=2.03, 95%CI=1.10-3.77) and LNM (OR=2.83, 95%CI=1.20-4.71). Additionally, patients lacking progesterone receptor exhibited high expression of MMP-11 and CK-20.

CONCLUSION:

We demonstrate that MMP-11 and CK-20 are probable prognostic markers whose expression reflects the stages of tumor differentiation and LNM of breast cancer.

• Authors: Chun-Wen Cheng, Jyh-Cherng Yu, Hsiao-Wei Wang, Chiun-Sheng Huang, Jia-Ching Shieh, Yi-Ping Fu, Chia-Wei Chang, Pei-Ei Wu, Chen-Yang Shen
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 411 Issue 3-4 Pg. 234-41 (Feb 2010) ISSN: 1873-3492 [Electronic] Netherlands
• PMID: 19914229 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2009 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies
• Biomarkers, Tumor
• Keratin-20
• RNA, Messenger
• Receptors, Estrogen
• Receptors, Progesterone
• Matrix Metalloproteinase 11

Topics

• Adult
• Aged
• Antibodies (immunology)
• Biomarkers, Tumor (genetics)
• Breast Neoplasms (diagnosis, genetics, metabolism, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• Genetic Predisposition to Disease
• Humans
• Immunohistochemistry
• Keratin-20 (genetics)
• Lymphatic Metastasis (genetics)
• Matrix Metalloproteinase 11 (genetics, immunology)
• Middle Aged
• Neoplasm Staging
• Prognosis
• RNA, Messenger (genetics, metabolism)
• Receptors, Estrogen (metabolism)
• Receptors, Progesterone (metabolism)