Abstract | PURPOSE: METHODS: The participants comprised of a high myopia group (n=194; age range, 17-24 years) having a myopic spherical equivalent greater than 6.5 diopters (D) and a control group (n=109; age range, 17-25 years) having a myopic spherical equivalent less than 0.5 D. Genotyping was performed using an assay-on-demand allelic discrimination assay. Polymerase chain reaction (PCR) was performed using 96 well plates on a thermal cycler. The polymorphisms detected were S1 (CHRM1rs11823728), S2 (CHRM1rs544978), S3 (CHRM1rs2186410), and S4 (CHRM1rs542269). RESULTS: There was a significant difference in the distribution of S2 and S4 between the high myopia and control groups (p=2.40 x 10(-6) and 2.38 x 10(-8), respectively). The odds ratios of AA genotype of S2 and GG genotype of S4 were both 0.08 (95% confidence interval [CI]: 0.02-0.29 and 0.02-0.36, respectively). Logistic regression test revealed S1, S2, and S4 CHRM1 as all being significant in the development of high myopia. Moreover, the distributions of haplotype 4 (Ht4; C/A/A/A) differed significantly between the two groups (p=3.4 x 10(-5), odds ratio: 0.1, 95% CI: 0.03-0.34). CONCLUSIONS: Our results suggest that the S2 and S4 polymorphisms of CHRM1 are associated with susceptibility for developing high myopia. S1, S2, and S4 CHRM1 had a co-operative association with high myopia.
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Authors | Hui-Ju Lin, Lei Wan, Yuhsin Tsai, Wen-Chi Chen, Shih-Wei Tsai, Fuu-Jen Tsai |
Journal | Molecular vision
(Mol Vis)
Vol. 15
Pg. 1774-80
(Sep 04 2009)
ISSN: 1090-0535 [Electronic] United States |
PMID | 19753311
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CHRM1 protein, human
- Receptor, Muscarinic M1
- Receptors, Muscarinic
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Topics |
- Confidence Intervals
- Female
- Gene Frequency
- Genetic Predisposition to Disease
- Haplotypes
- Humans
- Male
- Myopia
(genetics)
- Odds Ratio
- Polymorphism, Single Nucleotide
(genetics)
- Receptor, Muscarinic M1
- Receptors, Muscarinic
(genetics)
- Regression Analysis
- Young Adult
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