Tylophorine arrests carcinoma cells at G1 phase by downregulating cyclin A2 expression.

Abstract

Tylophorine, a representative phenanthroindolizidine alkaloid from Tylophoraindica plants, exhibits anti-inflammatory and anti-cancerous growth activities. However, the underlying mechanisms of its anti-cancer activity have not been elucidated and its effects on cell cycle remain ambiguous. Here, we reveal by asynchronizing and synchronizing approaches that tylophorine not only retards the S-phase progression but also dominantly arrests the cells at G1 phase in HepG2, HONE-1, and NUGC-3 carcinoma cells. Moreover, tylophorine treatment results in down regulated cyclin A2 expression and overexpressed cyclin A2 rescues the G1 arrest by tylophorine. Thus, we are the first to report that the downregulated cyclin A2 plays a vital role in G1 arrest by tylophorine in carcinoma cells.

• Authors: Chia-Mao Wu, Cheng-Wei Yang, Yue-Zhi Lee, Ta-Hsien Chuang, Pei-Lin Wu, Yu-Sheng Chao, Shiow-Ju Lee
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 386 Issue 1 Pg. 140-5 (Aug 14 2009) ISSN: 1090-2104 [Electronic] United States
• PMID: 19501048 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alkaloids
• Antineoplastic Agents
• CCNA2 protein, human
• Cyclin A
• Cyclin A2
• Indolizines
• Phenanthrenes
• tylophorine

Topics

• Alkaloids (pharmacology)
• Antineoplastic Agents (pharmacology)
• Carcinoma (metabolism)
• Cell Line, Tumor
• Cyclin A (antagonists & inhibitors, genetics)
• Cyclin A2
• Down-Regulation
• G1 Phase (drug effects)
• Gene Expression Regulation, Neoplastic
• Humans
• Indolizines (pharmacology)
• Phenanthrenes (pharmacology)
• Transcription, Genetic (drug effects)