Depletion of the reduced form of
glutathione (GSH) has been extensively studied for its effect on sensitizing
cancer to radiation. However, little is known about the effects of
thiol oxidative stress created through an increase in
glutathione disulfide (
GSSG) on
cancer sensitivity to radiation. In this study, an increase in
GSSG was effectively created using 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]
propionic acid (2-AAPA), an irreversible
glutathione reductase (GR) inhibitor. Our results demonstrate that the
GSSG increase significantly enhanced
cancer sensitivity to X-ray irradiation in four human
cancer cell lines (A431, MCF7, NCI-H226, and OVCAR-3). When cells were pretreated with 2-AAPA followed by X-ray irradiation, the IC(50) values for X-ray irradiation of A431, MCF7, NCI-H226, and OVCAR-3 cells were reduced, from 24.2 +/- 2.8, 42.5 +/- 3.0, 43.0 +/- 3.6, and 27.8+/-3.5 Gy to 6.75 +/- 0.9, 8.1 +/- 1.1, 6.75 +/- 1.0, and 12.1 +/- 1.7 Gy, respectively. The synergistic effects observed from the combination of X-rays plus 2-AAPA were comparable to those from the combination of X-rays plus
buthionine sulfoximine, a reference compound known to increase
cancer sensitivity to radiation. The synergistic effect was correlated with an increase in cell
thiol oxidative stress, which was reflected by a five-to sixfold increase in
GSSG and 25% increase in total
disulfides. No change in GSH or total
thiols was observed as a result of GR inhibition.