Endothelial nitric oxide synthase activation contributes to post-exercise hypotension in spontaneously hypertensive rats.

Abstract

We investigated the role that endothelial nitric oxide synthase plays in post-exercise hypotension in spontaneously hypertensive rats. To accomplish this, rats were subjected to a single bout of dynamic exercise on a treadmill at 15 m/min for 20 min. L-nitroarginine methyl ester (L-NAME, 40 mg/kg, i.p.) significantly inhibited post-exercise hypotension (25+/-11 and 5+/-3 mm Hg, respectively; P<0.05). In addition, the superoxide anion generation was decreased, while the plasma nitrite production and serine phosphorylation of endothelial nitric oxide synthase were significantly elevated in spontaneously hypertensive rats at 30 min after the termination of exercise. Taken together, these data demonstrate that the increased phosphorylation of endothelial nitric oxide synthase plays a crucial role in the reduction of arterial pressure following a single bout of dynamic exercise in spontaneously hypertensive rats.

• Authors: Sang Ki Lee, Cuk Seong Kim, Hyo Shin Kim, Eun Jung Cho, Hee Kyoung Joo, Ji Young Lee, Eun Ji Lee, Jin Bong Park, Byeong Hwa Jeon
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 382 Issue 4 Pg. 711-4 (May 15 2009) ISSN: 1090-2104 [Electronic] United States
• PMID: 19306842 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Nitric Oxide Synthase Type III
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Enzyme Activation
• Enzyme Inhibitors (pharmacology)
• Hypotension (enzymology)
• Male
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide Synthase Type III (antagonists & inhibitors, metabolism)
• Phosphorylation
• Physical Conditioning, Animal
• Rats
• Rats, Inbred SHR