Chromophobe renal cell carcinoma: useful diagnostic application of imprint cytology and fluorescence in situ hybridization of chromosomes 10 and 21 in two cases of typical and eosinophilic variants.

Abstract

Chromophobe renal cell carcinoma (RCC) is subdivided into typical and eosinophilic variants. We report such two cases with focus on imprint cytology and fluorescence in situ hybridization (FISH). The first case is a 53-year-old Japanese man and the second is a 76-year-old Japanese man. Histologically, the diagnosis of typical and eosinophilic variants of chromophobe RCC was suspected. In imprint cytology, irregularity of nuclear membrane, binucleation, perinuclear halo, and thick cell border were observed. Immunohistochemically, neoplastic cells of both tumors were positive for cytokeratin 7, E-cadherin, c-kit, and CD10. In FISH study, both tumors revealed the monosomy of chromosomes 10 and 21. Additionally, FISH study in eosinophilic variant of chromophobe RCC showed the disomy of chromosomes 7 and 17. In conclusion, we suggest that the combination study of imprint cytology and FISH of chromosomes 10 and 21 as well as routine histology may contribute to the accurate diagnosis of chromophobe RCC.

• Authors: Naoto Kuroda, Kazunobu Katto, Tadanori Yamaguchi, Takako Kawada, Yoshiaki Imamura, Ondrej Hes, Michal Michal, Taro Shuin, Gang-Hong Lee
• Journal: Medical molecular morphology (Med Mol Morphol) Vol. 41 Issue 4 Pg. 227-32 (Dec 2008) ISSN: 1860-1480 [Print] Japan
• PMID: 19107613 (Publication Type: Case Reports, Journal Article)

Topics

• Carcinoma, Renal Cell (classification, diagnosis, genetics, pathology)
• Chromosomes, Human, Pair 10
• Chromosomes, Human, Pair 17
• Chromosomes, Human, Pair 21
• Chromosomes, Human, Pair 7
• Cytological Techniques
• Eosinophils
• Humans
• In Situ Hybridization, Fluorescence
• Japan
• Kidney Neoplasms (classification, diagnosis, genetics, pathology)
• Male
• Middle Aged