Abstract |
A method based on reversed-phase liquid chromatography (HPLC) has been developed for the simultaneous identification and quantitative determination of four anti- dandruff agents such as salicylic acid, ketoconazole, climbazole, octopirox in commercial anti- dandruff shampoo products. A symmetry C18 column (5 microm, 250 mm x 4.6 mm i.d.) was used at temperature of 35 degrees C, mobile phase with flow rate of 0.8 mL min(-1) was acetonitrile: water (containing 10 mm potassium dihydrogen phosphate, pH 4.0, adjusted with orthophosphoric acid) = 60 : 40 (V/V) and UV detection at 224 nm and 305 nm. Samples were extracted with mobile phase by stirring and ultrasonic method. The average recoveries of four anti- dandruff agents were 98.0-104.1%. The relative standard deviations for samples were 0.11-0.90%. The method is simple, rapid and reproducible.
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Authors | L Chao |
Journal | International journal of cosmetic science
(Int J Cosmet Sci)
Vol. 23
Issue 3
Pg. 183-8
(Jun 2001)
ISSN: 1468-2494 [Electronic] England |
PMID | 18498472
(Publication Type: Journal Article)
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