[Detection of human cyclin C gene expression in childhood acute lymphocytic leukemia using real-time fluorescence quantitative PCR].

Abstract

OBJECTIVE:

To explore the relationship between human cyclin C (CCNC) gene and childhood acute lymphocytic leukemia (ALL).

METHODS:

The total RNA isolated from myeloid tissues of normal children and of children with newly diagnosed ALL and from ALL cell line 6T-CEM was reversely transcribed into cDNA. Real-time fluorescence quantitative PCR method was used to detect CCNC gene expression.

RESULTS:

CCNC was expressed in myeloid tissues of normal children and of children with newly diagnosed ALL as well as 6T-CEM. The relative expression level of CCNC gene in children with newly diagnosed ALL was significantly lower than in normal controls (2.35 +/- 0.83 vs 13.5 +/- 0.30; P <0.05).

CONCLUSIONS:

CCNC gene shows lower expression in children with newly diagnosed ALL, suggesting that it may be a tumor suppressing gene in childhood ALL.

• Authors: Zhao-Xia Zhang, Li-Zhi Cao, Qiong Huang, Ming-Hua Yang, Zhuo Wang, Yan Yu
• Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics (Zhongguo Dang Dai Er Ke Za Zhi) Vol. 10 Issue 1 Pg. 14-6 (Feb 2008) ISSN: 1008-8830 [Print] China
• PMID: 18289462 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCNC protein, human
• Cyclin C
• Cyclins

Topics

• Child
• Cyclin C
• Cyclins (genetics)
• Female
• Fluorescence
• Humans
• Male
• Polymerase Chain Reaction (methods)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (metabolism)