Evidence that intracoronary-injected CD133+ peripheral blood progenitor cells home to the myocardium in chronic postinfarction heart failure.

Abstract

OBJECTIVE:

To study the biodistribution of purified CD133(+) cells after intracoronary injection in patients with stable chronic postinfarction heart failure.

PATIENTS AND METHODS:

Patients with longstanding myocardial infarction (>12 months prior to inclusion) and with an accessible left coronary artery were eligible. CD133(+) cells were mobilized with granulocyte colony-stimulating factor and purified with a CliniMACS device. Cells were labeled with (111)Indium and injected through a balloon catheter in a coronary artery feeding the necrotic or viable infarct-related region of the left ventricle during a standard coronary catheterization procedure. The total body biodistribution of (111)Indium was studied with a dual-head gamma camera in combination with (99m)Technetium-sestaMIBI cardiac distribution analysis.

RESULTS:

The number of CD133(+) cells injected ranged between 5 and 10 x 10(6) cells (low dose, three patients) or between 18.5 and 50 x 10(6) cells (high dose, five patients). In the five patients receiving the higher cell doses, a clear residual radioactivity was observed at the level of the chronic injury at 2, 12, and up to 36 hours after injection. A detailed analysis in two patients showed 6.9% to 8.0% (after 2 hours) and 2.3% to 3.2% (after 12 hours) residual radioactivity at the heart. No adverse events were observed during the procedure and up to 3 months follow-up.

CONCLUSIONS:

We demonstrate that CD133(+) progenitor cells are capable of homing to the postinfarction remodeling myocardium after intracoronary injections in patients with chronic postinfarction heart failure.

• Authors: Rik Schots, Gilles De Keulenaer, Danny Schoors, Vicky Caveliers, Martine Dujardin, Stefan Verheye, Guy Van Camp, Philippe R Franken, Jan Roland, Ivan Van Riet, Hendrik Everaert
• Journal: Experimental hematology (Exp Hematol) Vol. 35 Issue 12 Pg. 1884-90 (Dec 2007) ISSN: 0301-472X [Print] Netherlands
• PMID: 17923244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AC133 Antigen
• Antigens, CD
• Glycoproteins
• PROM1 protein, human
• Peptides

Topics

• AC133 Antigen
• Aged
• Antigens, CD (immunology)
• Chronic Disease
• Female
• Glycoproteins (immunology)
• Heart Failure (pathology)
• Humans
• Male
• Middle Aged
• Myocardial Infarction (immunology, pathology)
• Myocardium (pathology)
• Peptides (immunology)
• Stem Cells (cytology, immunology)