Abstract |
Specific chromosomal abnormalities such as chromosome 13 deletions and some translocations affecting the immunoglobulin heavy chain (IGH) gene, namely t(4;14)(p16;q32) and t(14;16)(q32;q23) have been associated with an adverse prognosis in multiple myeloma. Conventional cytogenetic techniques fail to detect these aberrations in the majority of cases. Thus, we have developed a novel set of interphase fluorescence in situ hybridization (I-FISH) assays targeting those regions frequently lost on chromosome 13 as well as those oncogenes most recurrently involved in translocations with the IGH locus in multiple myeloma, i.e. IRTA1/2 (1q21), FGFR3/MMSET (4p16), CCND3 (6p21), IRF4 (6p25), CCND1 (11q13), MAF (16q23), and MAFB (20q12). The probes were combined in a multicolor fashion to develop novel multicolor I-FISH (MI-FISH) assays, whose validity and applicability was evaluated in negative controls and in a series of 13 plasma cell neoplasias. Additionally, a combination of the novel MI-FISH assays with staining for the plasma cell-specific antigen VS38c by means of multicolor FICTION (M-FICTION, fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms) allowed us to selectively analyze the plasma cell compartment, and thereby to increase the assay sensitivity.
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Authors | Borja Sáez, José I Martín-Subero, María D Odero, Felipe Prosper, Juan C Cigudosa, Robert Schoch, María J Calasanz, Reiner Siebert |
Journal | Oncology reports
(Oncol Rep)
Vol. 18
Issue 5
Pg. 1099-106
(Nov 2007)
ISSN: 1021-335X [Print] Greece |
PMID | 17914559
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin Heavy Chains
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Topics |
- Aged
- Chromosomes, Human, Pair 11
(genetics)
- Chromosomes, Human, Pair 14
(genetics)
- Cytogenetic Analysis
- Female
- Humans
- Immunoglobulin Heavy Chains
(genetics)
- In Situ Hybridization, Fluorescence
- Interphase
(genetics)
- Male
- Middle Aged
- Multiple Myeloma
(genetics, pathology)
- Paraproteinemias
(genetics, pathology)
- Plasma Cells
(pathology)
- Translocation, Genetic
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