[Development of methods for detection of H5N1 from human clinical specimens].

Abstract

BACKGROUND:

To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.

METHODS:

The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.

RESULTS:

The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.

CONCLUSION:

The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.

• Authors: Le-ying Wen, Hong Xu, Yu Lan, Xiang Zhao, Xiao-guang Zhang, Da-yan Wang, Li-hong Yao, Jie Dong, Jia-huai Zhang, Yuan-ji Guo, Yue-long Shu
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 20 Issue 2 Pg. 24-6 (Jun 2006) ISSN: 1003-9279 [Print] China
• PMID: 16816856 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Viral Proteins

Topics

• Animals
• Chick Embryo
• DNA Primers (genetics)
• Humans
• Influenza A Virus, H5N1 Subtype (genetics, isolation & purification)
• Influenza, Human (diagnosis, virology)
• Reproducibility of Results
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Sensitivity and Specificity
• Viral Proteins (genetics)