Amphotericin B tissue distribution in autopsy material after treatment with liposomal amphotericin B and amphotericin B colloidal dispersion.

Abstract

OBJECTIVES:

Tissue concentrations of amphotericin B were determined in autopsy material of patients who had been treated with liposomal amphotericin B or amphotericin B colloidal dispersion (colloidal amphotericin B) for suspected or proven invasive fungal infection.

PATIENTS AND METHODS:

Amphotericin B tissue levels were measured in liver, spleen, lung, kidney, and myocardial and brain tissue of 20 patients who had been treated with lipid-formulated amphotericin B, before they died from multi-organ failure. Seven patients had been treated with liposomal amphotericin B (AmBisome) and thirteen with colloidal amphotericin B (Amphocil). Tissue samples were obtained during routine autopsy, homogenized and extracted with methanol. Amphotericin B concentrations were measured using HPLC after purification by solid phase extraction.

RESULTS:

The highest amphotericin B levels were found in liver and spleen, followed by kidney, lung, myocardium and brain. In the lung higher amphotericin B concentrations were found after treatment with amphotericin B colloidal dispersion than after therapy with liposomal amphotericin B.

CONCLUSIONS:

The choice of lipid formulation may influence amphotericin B penetration into the lung.

• Authors: Helene Vogelsinger, Stefan Weiler, Angela Djanani, Jordan Kountchev, Rosa Bellmann-Weiler, Christian J Wiedermann, Romuald Bellmann
• Journal: The Journal of antimicrobial chemotherapy (J Antimicrob Chemother) Vol. 57 Issue 6 Pg. 1153-60 (Jun 2006) ISSN: 0305-7453 [Print] England
• PMID: 16627591 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tissue Extracts
• liposomal amphotericin B
• Amphotericin B

Topics

• Adult
• Aged
• Amphotericin B (analysis, pharmacokinetics)
• Autopsy
• Brain Chemistry
• Chromatography, High Pressure Liquid
• Female
• Humans
• Kidney (chemistry)
• Liver (chemistry)
• Lung (chemistry)
• Male
• Middle Aged
• Multiple Organ Failure
• Mycoses (complications, drug therapy)
• Myocardium (chemistry)
• Spleen (chemistry)
• Tissue Distribution
• Tissue Extracts (chemistry, isolation & purification)