Combined carriership of TLR9-1237C and CD14-260T alleles enhances the risk of developing chronic relapsing pouchitis.

Abstract

AIM:

To investigate the single nucleotide polymorphisms (SNPs) in genes involved in bacterial recognition and the susceptibility to pouchitis or pouchitis severity.

METHODS:

Analyses of CD14 -260C>T, CARD15/NOD2 3020insC, Toll-like receptor (TLR)4 +896A>G, TLR9 -1237T>C, TLR9+2848G>A, and IRAKM + 22148G>A SNPs were performed in 157 ileal-pouch anal anastomosis (IPAA) patients (79 patients who did not develop pouchitis, 43 infrequent pouchitis patients, 35 chronic relapsing pouchitis patients) and 224 Italian Caucasian healthy controls.

RESULTS:

No significant differences were found in SNP frequencies between controls and IPAA patients. However, a significant difference in carriership frequency of the TLR9-1237C allele was found between the infrequent pouchitis and chronic relapsing pouchitis groups [P = 0.028, oddos ratio (OR) = 3.2, 95%CI = 1.2-8.6]. This allele uniquely represented a 4-locus TLR9 haplotype comprising both studied TLR9 SNPs in Caucasians. Carrier trait analysis revealed an enhanced combined carriership of the alleles TLR9 -1237C and CD14 -260T in the chronic relapsing pouchitis and infrequent pouchitis group (P = 0.018, OR = 4.1, 95%CI = 1.4 -12.3).

CONCLUSION:

There is no evidence that the SNPs predispose to the need for IPAA surgery. The significant increase of the combined carriership of the CD14 -260T and TLR9 -1237C alleles in the chronic relapsing pouchitis group suggests that these markers identify a subgroup of IPAA patients with a risk of developing chronic or refractory pouchitis.

• Authors: K M Lammers, S Ouburg, S A Morré, J B A Crusius, P Gionchett, F Rizzello, C Morselli, E Caramelli, R Conte, G Poggioli, M Campieri, A S Peña
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 11 Issue 46 Pg. 7323-9 (Dec 14 2005) ISSN: 1007-9327 [Print] United States
• PMID: 16437636 (Publication Type: Journal Article)

Chemical References

• Lipopolysaccharide Receptors
• TLR9 protein, human
• Toll-Like Receptor 9
• DNA

Topics

• Adult
• Alleles
• Base Sequence
• Case-Control Studies
• Chronic Disease
• Colonic Pouches (adverse effects)
• DNA (genetics)
• Female
• Gene Frequency
• Haplotypes
• Humans
• Lipopolysaccharide Receptors (genetics)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Pouchitis (etiology, genetics, immunology)
• Recurrence
• Risk Factors
• Toll-Like Receptor 9 (genetics)