Kir6.2-deficient mice are susceptible to stimulated ANP secretion: K(ATP) channel acts as a negative feedback mechanism?

Abstract

OBJECTIVE:

While atrial natriuretic peptide (ANP) has been shown to be released mainly from cardiac muscle cells in response to atrial distension, the regulatory mechanisms of ANP secretion are still not fully understood. We sought to determine whether the ATP-sensitive K+ (K(ATP)) channel modulates the secretion of ANP, using mice with homozygous knockout of the Kir6.2 (a pore-forming subunit of cardiac K(ATP) channel) gene.

METHODS:

K(ATP) channel currents were recorded from isolated mouse atrial cells with patch-clamp techniques. Plasma ANP concentrations in anesthetized mice and ANP content and secretion in isolated atrial preparations were determined by radioimmunoassay. Action potentials were recorded from the isolated atria.

RESULTS:

Exposure to 2,4-dinitrophenol (100 microM) evoked a glibenclamide-sensitive K(ATP) channel current in atrial cells from wild-type (WT) but not Kir6.2 knockout (Kir6.2 KO) mice. Although there were no significant differences in the basal plasma ANP levels between WT and Kir6.2 KO mice, volume expansion caused a significant elevation of plasma ANP concentration in Kir6.2 KO but not WT mice with accompanying hypotension. When isolated left atria were stretched, ANP secreted into the bath from Kir6.2 KO atria was significantly higher than that from WT atria. Furthermore, stretching the atria from WT but not Kir6.2 KO mice significantly shortened the action potential duration. A hypotonic stretch of the membrane induced the glibenclamide-sensitive K(ATP) channel current in atrial cells from WT but not Kir6.2 KO mice.

CONCLUSIONS:

Kir6.2 is essential for the function of K(ATP) channel in mouse atrial cells. Given that Kir6.2 KO mice are susceptible to stretch-induced secretion of ANP, our results suggest that K(ATP) channels may act as a negative feedback mechanism for the control of ANP secretion.

• Authors: Noriko Saegusa, Toshiaki Sato, Tomoaki Saito, Masaji Tamagawa, Issei Komuro, Haruaki Nakaya
• Journal: Cardiovascular research (Cardiovasc Res) Vol. 67 Issue 1 Pg. 60-8 (Jul 01 2005) ISSN: 0008-6363 [Print] England
• PMID: 15949470 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Kir6.2 channel
• Potassium Channels
• Potassium Channels, Inwardly Rectifying
• Atrial Natriuretic Factor

Topics

• Action Potentials
• Animals
• Aorta
• Atrial Natriuretic Factor (analysis, metabolism)
• Cells, Cultured
• Feedback, Physiological
• Heart Atria
• Mice
• Mice, Knockout
• Myocytes, Cardiac (metabolism)
• Patch-Clamp Techniques
• Potassium Channels (metabolism)
• Potassium Channels, Inwardly Rectifying (genetics)
• Stimulation, Chemical