Matrix metalloproteinase-9 in bronchiolitis obliterans syndrome after lung transplantation.

Abstract

Bronchiolitis obliterans syndrome (BOS) is a severe complication after lung transplantation (LTX). In a retrospective cohort study 12 stable healthy recipients (non-BOS) and eight patients with BOS were enrolled after LTX and matrix metalloproteinases (MMP)-9, TIMP-1 and cell characteristics in bronchoalveolar lavage (BAL) samples (n = 145) were analysed. BALs from patients with BOS were further divided according to whether they were obtained before (pre-BOS) or after manifestation of BOS (BOS group). The MMP-9/TIMP-1 ratio was significantly increased in the BOS group compared with non-BOS or pre-BOS; furthermore, the ratio was negatively correlated with forced expiratory volume in one second. In zymography, the active form of MMP-9 was detected predominantly in the BOS group. In addition, zymography showed the banding pattern of neutrophil-derived MMP-9, indicating that polymorphonuclear neutrophils (PMNs) were the main source of MMP-9. According to that, MMP-9 was significantly correlated with the number of PMN. In immunocytochemistry, MMP-9 was also associated predominantly with PMN. This is the first study to evaluate the expression of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinases-1 over time during manifestation of a fibroproliferative lung disease in patients. It demonstrates development of bronchiolitis obliterans syndrome after lung transplantation is associated with an imbalance of matrix metalloproteinases-9/tissue inhibitors of metalloproteinase-1 ratio.

• Authors: R H Hübner, S Meffert, U Mundt, H Böttcher, S Freitag, N E El Mokhtari, T Pufe, S Hirt, U R Fölsch, B Bewig
• Journal: The European respiratory journal (Eur Respir J) Vol. 25 Issue 3 Pg. 494-501 (Mar 2005) ISSN: 0903-1936 [Print] England
• PMID: 15738294 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Tissue Inhibitor of Metalloproteinase-1
• Matrix Metalloproteinase 9

Topics

• Adult
• Bronchiolitis Obliterans (enzymology, etiology)
• Bronchoalveolar Lavage Fluid (chemistry)
• Cell Count
• Cohort Studies
• Female
• Humans
• Lung Transplantation (adverse effects)
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Middle Aged
• Neutrophils (metabolism)
• Reference Values
• Respiratory Function Tests
• Retrospective Studies
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)