Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX.

Abstract

We produced a novel cationic-charged modified DNA aptamer for sialyllactose that is a ubiquitous component of the cell surface responsible for the infection of several viruses by using the magnetic-particle-based SELEX method. After 13 rounds of selection we selected 22 clones as sialyllactose-binding DNA aptamers composed of several modified thymidines. The DNA aptamers could form a three-way junction structure that likely forms a binding site for siallyllactose. The three-way junction structure contains several modified thymidines bearing a positively-charged amino group at the C5 position, which could enhance the binding ability for silalyllactose which has a negatively-charged carboxyl group. The dissociation constant of the aptamer that showed the strongest sialyllactose-binding ability among the clones of the aptamers was 4.9 microM.

• Authors: Mohammad Mehedi Masud, Masayasu Kuwahara, Hiroaki Ozaki, Hiroaki Sawai
• Journal: Bioorganic & medicinal chemistry (Bioorg Med Chem) Vol. 12 Issue 5 Pg. 1111-20 (Mar 01 2004) ISSN: 0968-0896 [Print] England
• PMID: 14980623 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 2,6-sialyllactose
• Membrane Glycoproteins
• DNA
• Lactose
• Thymidine

Topics

• Binding Sites
• DNA (chemistry, isolation & purification, metabolism)
• Gene Library
• Lactose (analogs & derivatives, metabolism)
• Membrane Glycoproteins (antagonists & inhibitors, chemistry)
• Models, Molecular
• Nucleic Acid Conformation
• Thymidine (analogs & derivatives, chemistry)