[Interactions between lifestyle, methylanetetrahydrofolate reductase gene and polymorphisms in thymidylate synthase gene with risk of stomach cancer].

Abstract

OBJECTIVE:

To evaluate interactions between lifestyle, methylanetetrahydrofolate reductase gene (MTHFR) and polymorphisms in the 3'-untranslated region (3'-UTR) of the thymidylate synthase gene (TS) with reference to development of stomach cancer (SC).

METHODS:

We conducted a case-control study with 107 cases of SC and 200 population-based controls in Huaian city of Jiangsu province, China. TS genotypes were identified by polymerase chain reaction.

RESULTS:

(1) The frequencies of TS genotypes (+6 bp/+6 bp, +6 bp/-6 bp and -6 bp/-6 bp) among the cases were 5.6%, 47.7% and 46.7% and among the controls were 9.0%, 54.0% and 37.0%, respectively. Individuals identified as -6 bp/-6 bp genotype had a slightly higher risk for SC than those individuals with +6 bp alleles (the crude OR = 1.49, 95% CI: 0.90 - 2.47; adjusted OR = 1.36, 95% CI: 1.00 - 1.78, P = 0.047). (2) Individuals having TS -6 bp/-6 bp genotype and having smoking habit were at a significantly higher risk of developing SC (adjusted OR = 2.79, 95% CI: 1.51 - 5.18) compared with those who had +6 bp alleles with no smoking habit. Individuals having TS -6 bp/-6 bp genotype and habit of frequent alcohol drinking were at an increased risk of developing SC (adjusted OR = 1.76, 95% CI: 1.07 - 2.90) compared with those with +6 bp alleles and low consumption of alcohol. As compared with individuals with +6 bp alleles and who had habit of tea drinking, individuals who had TS -6 bp/-6 bp genotype and but without habit of tea drinking had an increased risk of developing SC (adjusted OR = 2.34, 95% CI: 1.43 - 3.82). (3) Individuals with TS -6 bp/-6 bp genotype and with MTHFR T alleles had an increased risk of developing SC (adjusted OR = 2.67, 95% CI: 1.07 - 6.70) compared with those with +6 bp alleles and with MTHRF C/C genotype.

CONCLUSION:

Results in the present study suggested that there was a combined effect between lifestyle, MTHFR C/T or T/T genotype and TS -6 bp/-6 bp genotype in the development of SC.

• Authors: Chang-ming Gao, Jian-zhong Wu, Yan-ting Liu, Jian-hua Ding, Su-ping Li, Ping Su, Xu Hu, Hai-tao Kai, Takezaki Toshiro, Tajima Kazuo
• Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (Zhonghua Liu Xing Bing Xue Za Zhi) Vol. 24 Issue 7 Pg. 599-603 (Jul 2003) ISSN: 0254-6450 [Print] China
• PMID: 12975018 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tea
• Methylenetetrahydrofolate Reductase (NADPH2)
• Thymidylate Synthase

Topics

• Alcohol Drinking (adverse effects)
• Case-Control Studies
• China (epidemiology)
• Female
• Genetic Predisposition to Disease
• Humans
• Life Style
• Male
• Methylenetetrahydrofolate Reductase (NADPH2) (genetics)
• Point Mutation
• Polymorphism, Genetic
• Risk Factors
• Smoking (adverse effects)
• Stomach Neoplasms (epidemiology, genetics)
• Tea (chemistry)
• Thymidylate Synthase (genetics)