[Detection of human papillomavirus and herpesvirus genotypes in biopsy specimens from cervical carcinoma by PCR-endonuclease cleavage].

Abstract

OBJECTIVE:

This study was to detect the human papillomavirus (HPV) and herpes simplex virus (HSV) genotypes in biopsy specimens of cervical carcinoma and genital verruca by PCR-endonuclease cleavage.

METHODS:

Biopsy samples of were obtained from 390 patients with cervical carcinoma, HPV and HSV genotypes were detected by PCR and endonuclease cleavage.

RESULTS:

The results indicated that HPV types 16, 18, 35 were found in 152 (38.9%) of 390 cervical carcinoma, in 56 (31.8%) of 176 cervical erosion, while HPV types 6, 11 were found in 80 (58.8%) of 136 acuminate erruca. The HSV-2 type were found in 135 (34.6%) of 390 cervical carcinoma and in 33 (18.7%) of 176 cervical erosion. The HPV 16, 18, 35 types and HSV-2 type found in normal cervical secretions of the control group all were 3.2%, There was significant difference between the test group of cervical carcinoma and the control group of normal cervical secretion (P < 0.001 by x 2 analysis). The restriction endonuclease analysis of these amplified products showed that positive rates of HPV 16, 18, 35 types and HSV-2 type from cervical carcinoma were 23.3%, 14.6%, 1.0% and 34.6%, respectively. While HPV 6, 11 types from acuminate verruca were 36.0% and 22.8% positive respectively.

CONCLUSION:

The results suggest that HPV 16, 18 types and HSV-2 type are associated with majority of the cervical carcinoma.

• Authors: L Li, Q Zhu, S Zheng
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 13 Issue 3 Pg. 235-8 (Sep 30 1999) ISSN: 1003-9279 [Print] China
• PMID: 12569753 (Publication Type: English Abstract, Journal Article)

Topics

• Adult
• Female
• Genotype
• Herpes Genitalis (virology)
• Humans
• Papillomaviridae (isolation & purification)
• Papillomavirus Infections
• Polymerase Chain Reaction
• Simplexvirus (isolation & purification)
• Tumor Virus Infections
• Uterine Cervical Erosion (virology)
• Uterine Cervical Neoplasms (virology)