The role of common gamma chain in human monocytes in vivo; evaluation from the studies of X-linked severe combined immunodeficiency (X-SCID) carriers and X-SCID patients who underwent cord blood stem cell transplantation.

Abstract

Expression of common gamma chain (gammac) on monocytes was studied in five carriers of X-linked severe combined immunodeficiency (X-SCID) and two X-SCID patients who underwent cord blood stem cell transplantation (CBSCT). Flow cytometric analysis revealed that both gammac-negative and positive monocytes co-existed in X-SCID carriers, whereas no gammac-negative T, B or NK cells were observed in them. Clonal analysis and reverse transcription polymerase chain reaction studies revealed that 13.2-45.0% of monocytes from these carriers expressed the mutant gammac message. X-SCID patients who received CBSCT persistently possessed the majority of gammac-negative monocytes with a good clinical course. These results, together, may indicate that gammac is not essential for monocyte development/function in vivo.

• Authors: Tadashi Ariga, Koji Yamaguchi, Jukei Yoshida, Akihiko Miyanoshita, Toshihide Watanabe, Tadashi Date, June-Ichi Miura, Satoru Kumaki, Naoto Ishii, Yukio Sakiyama
• Journal: British journal of haematology (Br J Haematol) Vol. 118 Issue 3 Pg. 858-63 (Sep 2002) ISSN: 0007-1048 [Print] England
• PMID: 12181059 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IL2RG protein, human
• Interleukin Receptor Common gamma Subunit
• RNA, Messenger
• Receptors, Interleukin-7

Topics

• Female
• Fetal Blood
• Flow Cytometry
• Genetic Linkage
• Hematopoietic Stem Cell Transplantation
• Heterozygote
• Humans
• Interleukin Receptor Common gamma Subunit
• Male
• Monocytes (metabolism)
• Mutation
• RNA, Messenger (genetics)
• Receptors, Interleukin-7 (blood, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Severe Combined Immunodeficiency (blood, genetics)
• X Chromosome