Anterior capsule dyes and labeled viscoelastic solutions to enhance contrast in EAS-1000 Scheimpflug images.

Abstract

PURPOSE:

To enhance contrast in images produced by the EAS-1000 to facilitate evaluation of the rheological properties of viscoelastic substances during simulated cataract surgery.

SETTING:

Department of Preclinical Ophthalmology, Scientific Affairs, Pharmacia AB, Uppsala, Sweden.

METHODS:

Cataract surgery was performed in 200 fresh porcine cadaver eyes and documented by EAS-1000 Scheimpflug photography. The light scattering caused by anterior capsule dyes, labeled irrigation solutions, and labeled viscoelastic solutions was statistically analyzed in the images.

RESULTS:

Visualization by light scattering was significantly enhanced by a fluorescein labeled viscoelastic solution and by an eosin-Y- or phloxine-B-dyed capsule (P < .05). A side effect of eosin Y and phloxine B was that the capsule became fragile, making it difficult to perform a continuous curvilinear capsulorhexis.

CONCLUSIONS:

Fluorescein-labeled viscoelastic solutions are recommended to enhance contrast in the images when retention capacity is evaluated. Light scattering from capsule dyeing facilitated anterior chamber depth measurements in the images.

• Authors: Jörgen B Holmén
• Journal: Journal of cataract and refractive surgery (J Cataract Refract Surg) Vol. 28 Issue 2 Pg. 337-45 (Feb 2002) ISSN: 0886-3350 [Print] United States
• PMID: 11821219 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Coloring Agents
• Contrast Media
• Drug Combinations
• Hyaluronic Acid

Topics

• Animals
• Anterior Chamber (anatomy & histology)
• Coloring Agents
• Contrast Media
• Drug Combinations
• Hyaluronic Acid
• Lens Capsule, Crystalline (anatomy & histology)
• Phacoemulsification
• Photography (methods)
• Staining and Labeling (methods)
• Swine