Hydrogen sulfide and colonic epithelial metabolism: implications for ulcerative colitis.

Abstract

Hydrogen sulfide (HS-) impairs the oxidation of butyrate in colonocytes and is found in excess in feces of patients with ulcerative colitis. The possible pathogenic role of HS- in ulcerative colitis was further investigated. To investigate the metabolic effect of free and bound fecal HS-, isolated rat colonocytes were incubated in the presence of butyrate without and with the addition of (1) HS- in water, (2) sterile filtrates of fecal homogenates supplemented and incubated with HS- and known sources of fecal HS- production, and (3) HS- incubated with fecal agents known to bind HS-. Oxidation rates were obtained by quantifying the production of CO2. Total and free HS-, as well as the fecal ability to bind HS-, were determined in health and ulcerative colitis. Compared to the production of CO2 by colonocytes incubated with 2 mmol/liter of butyrate, the further addition of 1.25 and 2.5 mmol/liter of HS- in water reduced the production of CO2 by 57.6+/-10.0 and 98.9+/-1.4%, respectively. However, when adding fecal filtrate of homogenate supplemented with HS- corresponding to 1.25 and 2.5 mmol/liter of HS- in water, the reduction of CO2 production was only 30.7+/-12.0 and 53.2+/-14.0%, respectively. Neither the fecal level of total or free HS- nor the remarkable fecal ability to bind HS- differed in health or quiescent and active ulcerative colitis. Bound HS- had no or little effect on CO2 production. Addition of fecal filtrate of nonsupplemented homogenate to colonocytes significantly reduced the oxidation of butyrate to CO2 about 25%, which could not be ascribed to fecal HS-. In conclusion, fecal HS- has little effect on butyrate oxidation in colonocytes and does not seem to play a pathogenic role for UC by impairing colonic epithelial metabolism. Other fecal agents seem to be more potent metabolic inhibitors than fecal HS-. The role of colonic contents in the pathogenesis of ulcerative colitis remains circumstantial.

• Authors: J Jørgensen, P B Mortensen
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 46 Issue 8 Pg. 1722-32 (Aug 2001) ISSN: 0163-2116 [Print] United States
• PMID: 11508674 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Butyrates
• Ketone Bodies
• Carbon Dioxide
• Hydrogen Sulfide

Topics

• Adult
• Animals
• Butyrates (pharmacology)
• Carbon Dioxide (metabolism)
• Colitis, Ulcerative (metabolism)
• Colon (cytology, metabolism)
• Feces (chemistry)
• Female
• Humans
• Hydrogen Sulfide (analysis, metabolism, pharmacology)
• In Vitro Techniques
• Intestinal Mucosa (metabolism)
• Ketone Bodies (metabolism)
• Male
• Middle Aged
• Oxidation-Reduction
• Rats
• Rats, Sprague-Dawley