Identification of larval-stage antigens of ascaris suum recognized with immune sera from pigs.

Abstract

Antigens were identified from the third-stage larvae (L3) and lung-stage larvae of Ascaris suum by two-dimensional immunoblot method with antisera obtained from pigs that received chemically abbreviated Ascaris suum larval infections. Forty-seven and 13 spots were recognized as antigens from the L3 and lung-stage larvae, respectively. Their apparent molecular weight ranged from 20 to 101 kDa and their isoelectric point from 3.6 to 8.0. The present study provides a framework for further molecular cloning of those antigens and consequently leads to the development of recombinant peptide vaccines against A. suum.

• Authors: H Kasug-Aoki, N Tsuji, K Suzuki, T Arakawa, Y Matsumoto, T Isobe
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 63 Issue 6 Pg. 683-5 (Jun 2001) ISSN: 0916-7250 [Print] Japan
• PMID: 11459018 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Helminth

Topics

• Animals
• Antigens, Helminth (analysis)
• Ascariasis (blood, immunology, parasitology, veterinary)
• Ascaris suum (growth & development, immunology)
• Blotting, Western (veterinary)
• Electrophoresis, Polyacrylamide Gel (veterinary)
• Female
• Isoelectric Focusing (veterinary)
• Male
• Specific Pathogen-Free Organisms
• Swine
• Swine Diseases (blood, immunology, parasitology)