Whole genome amplification and high-throughput allelotyping identified five distinct deletion regions on chromosomes 5 and 6 in microdissected early-stage ovarian tumors.

Abstract

Investigation of genetic changes in tumors by loss of heterozygosity is a powerful technique for identifying chromosomal regions that may contain tumor suppressor genes. In this study, we determined allelic loss on chromosomes 5 and 6 in 29 primary early-stage epithelial ovarian carcinomas including 3 microscopically identified adenocarcinomas using a high-throughput PCR-based method combined with laser capture microdissection and whole genome amplification techniques. Twenty microsatellite markers spanning chromosomes 5 and 6 at an average distance of 20 cM were examined. High frequencies of loss on chromosome 5 were identified at loci D5S428 (48%), D5S424 (32%), and D5S630 (32%). Our study also showed that chromosome 6 exhibited high frequencies of loss of heterozygosity at loci D6S1574 (46%), D6S287 (42%), D6S441 (45%), D6S264 (60%), and D6S281 (35%). These results suggest that multiple tumor suppressor genes are located on five distinct regions on chromosomes 5 and 6, i.e., 5p15.2, 5q13-21, 6p24-25, 6q21-23, and 6q25.1-27, and may be involved in the early development of ovarian carcinomas.

• Authors: V W Wang, D A Bell, R S Berkowitz, S C Mok
• Journal: Cancer research (Cancer Res) Vol. 61 Issue 10 Pg. 4169-74 (May 15 2001) ISSN: 0008-5472 [Print] United States
• PMID: 11358841 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Topics

• Adenocarcinoma (genetics, pathology)
• Adult
• Aged
• Alleles
• Chromosomes, Human, Pair 5 (genetics)
• Chromosomes, Human, Pair 6 (genetics)
• Dissection
• Female
• Gene Amplification
• Genes, Tumor Suppressor
• Humans
• Lasers
• Loss of Heterozygosity
• Microsatellite Repeats (genetics)
• Middle Aged
• Ovarian Neoplasms (genetics, pathology)
• Polymerase Chain Reaction