Tumor recurrence in patients with
cervical cancer after primary radical surgery with complete
tumor resection (R0) may be caused by occult
tumor cell deposits (OTCD) in potentially
tumor-bearing pelvic tissue. New surgical techniques, like
liposuction-assisted nerve-sparing radical abdominal
hysterectomy (LANS-RH) might be performed to remove this tissue. In a preliminary study of 30 cases, treated with LANS-RH, the
liposuction specimens were examined on
hematoxylin &
eosin (H&E)-stained step sections and at immunohistochemical levels, using the antibody cocktails MNF 116 and AE1/AE3 for cytokeratins to detect occult
tumor cells. In nine cases microscopically
tumor-free lymph nodes were detected in the
liposuction material. One case presented lymphovascular space involvement in the capsular region of a node on H&E stained slides. Two additional cases showed
tumor cell deposits in pelvic perinodal fatty tissue on immunohistochemical examination. We conclude that the LANS-RH technique is capable of resecting potentially
tumor-bearing pelvic tissue. Careful histologic handling and immunohistochemical techniques may improve the detection of OTCD.