Activation of antitumor cytotoxic T lymphocytes by fusions of human dendritic cells and breast carcinoma cells.

Abstract

We have reported that fusions of murine dendritic cells (DCs) and murine carcinoma cells reverse unresponsiveness to tumor-associated antigens and induce the rejection of established metastases. In the present study, fusions were generated with primary human breast carcinoma cells and autologous DCs. Fusion cells coexpressed tumor-associated antigens and DC-derived costimulatory molecules. The fusion cells also retained the functional potency of DCs and stimulated autologous T cell proliferation. Significantly, the results show that autologous T cells are primed by the fusion cells to induce MHC class I-dependent lysis of autologous breast tumor cells. These findings demonstrate that fusions of human breast cancer cells and DCs activate T cell responses against autologous tumors.

• Authors: J Gong, D Avigan, D Chen, Z Wu, S Koido, M Kashiwaba, D Kufe
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 97 Issue 6 Pg. 2715-8 (Mar 14 2000) ISSN: 0027-8424 [Print] United States
• PMID: 10688917 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Topics

• Breast Neoplasms (immunology, metabolism)
• Cell Culture Techniques (methods)
• Cell Fusion
• Dendritic Cells (cytology, immunology)
• Flow Cytometry
• Humans
• Immunohistochemistry
• K562 Cells
• Leukocytes, Mononuclear (metabolism)
• Lymphocyte Activation
• Phenotype
• T-Lymphocytes, Cytotoxic (immunology)
• Tumor Cells, Cultured