Identification of two lymphotoxin beta isoforms expressed in human lymphoid cell lines and non-Hodgkin's lymphomas.

Abstract

Two isoforms of lymphotoxin beta (LTbeta) were isolated from mRNAs of a panel of human lymphoid cell lines and tumor tissues obtained from patients with non-Hodgkin's lymphoma (NHL). The truncated LTbeta mRNA variant lacked 46 base pairs complementary to the complete sequence of exon 2, suggesting that both isoforms are produced by an alternative splicing mechanism. Skipping out of exon 2 causes a reading frame shift and a premature stop codon in the LTbeta mRNA variant. The predictive translated polypeptide would correspond to a severely shortened LTbeta protein that would lack the majority of the extracellular domain of the native molecule, thus impairing its normal complex assembly with LTalpha. These observations provide new insights into the molecular heterogeneity and biological function of LTbeta within the tumor necrosis factor and LT ligand-receptor system.

• Authors: K Warzocha, N Renard, C Charlot, J Bienvenu, B Coiffier, G Salles
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 238 Issue 2 Pg. 273-6 (Sep 18 1997) ISSN: 0006-291X [Print] United States
• PMID: 9299492 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 1997 Academic Press.

Chemical References

• LTB protein, human
• Lymphotoxin-alpha
• Lymphotoxin-beta
• Membrane Proteins
• RNA, Messenger

Topics

• Base Sequence
• Humans
• Lymphocytes (metabolism, pathology)
• Lymphoma, Non-Hodgkin (genetics, metabolism)
• Lymphotoxin-alpha (biosynthesis, genetics, isolation & purification)
• Lymphotoxin-beta
• Membrane Proteins (biosynthesis, genetics, isolation & purification)
• Molecular Sequence Data
• RNA, Messenger (analysis, biosynthesis, genetics)
• Sequence Analysis
• Tumor Cells, Cultured