Spin trapping agent phenyl N-tert-butylnitrone protects against the onset of drug-induced insulin-dependent diabetes mellitus.

Abstract

Insulin-dependent diabetes mellitus is an autoimmune disease believed to be caused by an inflammatory process in the pancreas leading to selective destruction of the beta-cells. Cytokines and nitric oxide (NO) have been shown to be involved in this destruction. Phenyl N-tert-butylnitrone (PBN) has demonstrated protective effects against several pathological conditions including ischemia-reperfusion injury and endotoxin-induced shock. We report here that PBN co-administration can prevent the onset of the STZ-induced diabetes in mice. PBN co-treatment inhibited the streptozotocin (STZ)-induced hyperglycemia, the elevation in the level of glycated hemoglobin and weight loss in the treated mice. Histological observations indicated destruction of B-cells in the STZ-treated animals and its prevention by PBN co-treatment. EPR spin trapping experiments in the pancreas indicated the in vivo formation of NO in STZ-treated animals and its attenuation by PBN treatment.

• Authors: T Tabatabaie, Y Kotake, G Wallis, J M Jacob, R A Floyd
• Journal: FEBS letters (FEBS Lett) Vol. 407 Issue 2 Pg. 148-52 (Apr 28 1997) ISSN: 0014-5793 [Print] England
• PMID: 9166889 (Publication Type: Journal Article)

Chemical References

• Cyclic N-Oxides
• Glycated Hemoglobin A
• Nitrogen Oxides
• phenyl-N-tert-butylnitrone
• Streptozocin

Topics

• Animals
• Cyclic N-Oxides
• Diabetes Mellitus, Experimental (prevention & control)
• Diabetes Mellitus, Type 1 (prevention & control)
• Electron Spin Resonance Spectroscopy
• Glycated Hemoglobin (analysis)
• Hyperglycemia (prevention & control)
• Islets of Langerhans (anatomy & histology)
• Male
• Mice
• Mice, Inbred BALB C
• Nitrogen Oxides (therapeutic use)
• Spin Trapping
• Streptozocin
• Weight Loss (drug effects)