Synthesis of thymine and alpha-putrescinylthymine in bacteriophage phi W-14-infected Pseudomonas acidovorans.

Abstract

Host DNA synthesis stopped about 10 min after the infection of Pseudomonas acidovorans with bacteriophage phi W-14, but host DNA was not degraded to acid-soluble fragments. The synthesis of host but not of phage DNA was inhibited by 5-fluorodeoxyuridine. The nucleotide pools of infected cells did not contain dTTP, and infection resulted in the appearance of dTTPase activity. Although ornithine labeled the alpha-putrescinylthymine residues of phi W-14 DNA, ornithine-labeled nucleotides were not detected in infected cells. A new deoxynucleoside triphosphate did appear in infected cells, but it was not labeled by ornithine. It is concluded that the thymine and alpha-putrescinylthymine in phi W-14 DNA are synthesized at the polynucleotide level.

• Authors: K L Maltman, J Neuhard, H A Lewis, R A Warren
• Journal: Journal of virology (J Virol) Vol. 34 Issue 2 Pg. 354-9 (May 1980) ISSN: 0022-538X [Print] United States
• PMID: 6445427 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Bacterial
• DNA, Viral
• Thymine Nucleotides
• Floxuridine
• alpha-putrescinylthymine
• Thymine
• Thymidine

Topics

• Bacteriophages (growth & development, metabolism)
• DNA, Bacterial (metabolism)
• DNA, Viral (biosynthesis)
• Floxuridine (pharmacology)
• Pseudomonas (metabolism)
• Thymidine (metabolism)
• Thymine (analogs & derivatives, biosynthesis)
• Thymine Nucleotides (metabolism)