The effect of L-buthionine sulfoximine on the aerobic radiation response of A549 human lung carcinoma cells.

Abstract

Our data show that A549 cells are increasingly radiosensitive with prolonged exposure to L-BSO. The resulting glutathione and protein thiol depleted cells show both loss of shoulder and slope modification. Furthermore, there is an increase in single strand DNA breaks and irrepairable cross-linking. The aerobic radiation damage in the thiol depleted state appears to be different from that obtained with hypoxic cells. Any postulated role for GSH in reducing or preventing peroxidative radiation damage must also include protection against single strand DNA breaks as well as involvement in repairing DNA-protein cross-links. The latter effect may be related to decreased protein thiol content as reflected in a decreased enzyme capacity to repair DNA damage.

• Authors: J E Biaglow, M E Varnes, S W Tuttle, N L Oleinick, K Glazier, E P Clark, E R Epp, L A Dethlefsen
• Journal: International journal of radiation oncology, biology, physics (Int J Radiat Oncol Biol Phys) Vol. 12 Issue 7 Pg. 1139-42 (Jul 1986) ISSN: 0360-3016 [Print] United States
• PMID: 3744931 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Radiation-Sensitizing Agents
• Methionine Sulfoximine
• Buthionine Sulfoximine
• Glutathione
• Oxygen

Topics

• Buthionine Sulfoximine
• Cell Line
• Cell Survival (drug effects, radiation effects)
• Glutathione (metabolism)
• Humans
• In Vitro Techniques
• Methionine Sulfoximine (analogs & derivatives, pharmacology)
• Oxygen (physiology)
• Radiation-Sensitizing Agents (pharmacology)
• Time Factors