Abstract | BACKGROUND: Broccoli has received widespread attention because of its anti-inflammatory and antioxidant effects. The present study aimed to explore the composition of broccoli seed extract (BSE) and its effect on colitis induced by dextran sulfate sodium (DSS). RESULTS: BSE mainly comprises glucoraphanin and polysaccharides composed of arabinose, galactose, glucose and mannose. Animal experiments suggested that BSE intervention effectively reversed body weight loss, suppressed the levels of proinflammatory interleukin-6, tumor necrosis factor-α and interleukin-1β, and elevated the levels of anti-inflammatory interleukin-10 and the activities of superoxide dismutase and glutathione in DSS-induced colitis mice. According to histopathologic and immunohistochemical analysis of colon tissue, BSE intervention may repair the intestinal barrier by upregulating mRNA levels and the expression of tight junction proteins ( claudin-1, occludin and zonula occludens-1). Gas chromatography-mass spectrometry (MS) analysis demonstrated that cecal short-chain fatty acids in mice with BSE administration were significantly increased compared with the model group. Sulforaphane and sulforaphane- N-acetylcysteine were only detected in BSE group mice by ultra-performance liquid chromatography-MS analysis. In addition, BSE intervention evidently increased the abundance of Alistipeds, Coriobacteriaceae UCG-002 and Bifidobacterium and decreased the abundance of Escheichia-Shinella, Lachnospiraceae others, Parabacteroides, Ruminococcaceae others and Turicibacter, which possibly promoted carbohydrate metabolism and short-chain fatty acid production. CONCLUSION: The present study aimed to elucidate the effect of BSE on colitis and found that BSE, as a novel food ingredient, has great potential for the improvement of colitis. © 2022 Society of Chemical Industry.
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Authors | Jiaying Wu, Weiling Guo, Shumao Cui, Xin Tang, Qiuxiang Zhang, Wenwei Lu, Yan Jin, Jianxin Zhao, Bingyong Mao, Wei Chen |
Journal | Journal of the science of food and agriculture
(J Sci Food Agric)
Vol. 103
Issue 4
Pg. 1749-1760
(Mar 15 2023)
ISSN: 1097-0010 [Electronic] England |
PMID | 36495024
(Publication Type: Journal Article)
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Copyright | © 2022 Society of Chemical Industry. |
Chemical References |
- sulforaphane
- glucoraphanin
- Anti-Inflammatory Agents
- Polysaccharides
- Plant Extracts
- Dextran Sulfate
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Topics |
- Animals
- Mice
- Brassica
- Gastrointestinal Microbiome
- Colitis
(chemically induced, drug therapy)
- Colon
- Anti-Inflammatory Agents
(pharmacology)
- Polysaccharides
(metabolism)
- Plant Extracts
(metabolism)
- Dextran Sulfate
(adverse effects)
- Mice, Inbred C57BL
- Disease Models, Animal
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