Abstract | Background: Objective: The objective of this study was the comparison of 2 methods for the analysis of real-time PCR (qPCR) data, through the use of the evaluation of genes that mediate the effect of Phycocyanobilin (PCB) and its validation in animal models. Methods: We evaluated the effect of PCB:" in vitro" on gene modulation through qPCR analyzed by parametric ANOVA and multivariate principal component analysis (PCA) in a model of glutamate-induced excitotoxicity in the SH-SY5Y cell line and" in vivo"; in animal models of multiple sclerosis (MS) and cerebral ischemia (CI). Results: The results showed that PCA is a robust and powerful method that allows the assessment of gene expression profiles. We detected the significant down-regulation of the CYBB (NOX2), and HMOX1 by the action of PCB in SH-5YSH cell line insulted with Glutamate. The decrease in pro-inflammatory cytokines and markers related to apoptosis and innate immune response, mediated the effect of PCB in the animal models of MS and CI, respectively. Conclusion: We concluded that the mechanisms by which PCB protected cells included the reduction of oxidative stress damage, which could contribute to its clinical efficacy for the treatment of neurodegenerative diseases.
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Authors | Daniel Palenzuela Gardón, Majel Cervantes-Llanos, Beatriz Piniella Matamoros, Hanlet Camacho Rodríguez, Chan-Yuan Tan, Javier Marín-Prida, Viviana Falcón-Cama, Nancy Pavón-Fuentes, Jessica Gómez Lemus, Laura de la Caridad Bakos Ruiz, Tamara Díaz Argudin, Gillian Martínez Donato, Yasser Perera, Ke Yang, Giselle Pentón-Rol |
Journal | Heliyon
(Heliyon)
Vol. 8
Issue 6
Pg. e09769
(Jun 2022)
ISSN: 2405-8440 [Print] England |
PMID | 35800718
(Publication Type: Journal Article)
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Copyright | © 2022 The Authors. |