Pattern recognition receptors (
PRRs) protect against microbial invasion by detecting specific molecular patterns found in pathogens and initiating an immune response. Although microbial-derived PRR
ligands have been extensively characterized, the contribution and relevance of endogenous
ligands to PRR activation remains overlooked. Here, we characterize the landscape of endogenous
ligands that engage RIG-I-like receptors (RLRs) upon
infection by different RNA viruses. In each
infection, several RNAs transcribed by
RNA polymerase III (Pol3) specifically engaged RLRs, particularly the family of Y RNAs. Sensing of Y RNAs was dependent on their mimicking of viral secondary structure and their 5'-triphosphate extremity. Further, we found that HIV-1 triggered a VPR-dependent downregulation of
RNA triphosphatase DUSP11 in vitro and in vivo, inducing a transcriptome-wide change of cellular
RNA 5'-triphosphorylation that licenses Y
RNA immunogenicity. Overall, our work uncovers the contribution of endogenous RNAs to
antiviral immunity and demonstrates the importance of this pathway in HIV-1
infection.