Microfluidic Production of Zwitterion Coating Microcapsules with Low Foreign Body Reactions for Improved Islet Transplantation.

Abstract

Islet transplantation is a promising strategy for type 1 diabetes mellitus (T1DM) treatment, whereas implanted-associated foreign body reaction (FBR) usually induces the necrosis of transplanted islets and leads to the failure of glycemic control. Benefiting from the excellent anti-biofouling property of zwitterionic materials and their successful application in macroscopic implanted devices, microcapsules with zwitterionic coatings may be promising candidates for islet encapsulation. Herein, a series of zwitterion-coated core-shell microcapsules is fabricated (including carboxybetaine methacrylate [CBMA]-coated gelatin methacrylate [GelMA] [CBMA-GelMA], sulfobetaine methacrylate [SBMA]-coated GelMA [SBMA-GelMA], and phosphorylcholine methacrylate [MPC]-coated GelMA [MPC-GelMA]) by one-step photopolymerization of inner GelMA and outer zwitterionic monomers via a handmade two-fluid microfluidic device and it is demonstrated that they can effectively prevent protein adsorption, cell adhesion, and inflammation in vitro. Interestingly, the zwitterionic microcapsules successfully resist FBR in C57BL/6 mice after intraperitoneal implantation for up to 4 months. After successfully encapsulating xenogeneic rat islets in the SBMA-GelMA microcapsules, sustained normoglycemia is further validated in streptozotocin (STZ)-induced mice for up to 3 months. The zwitterion-modified microcapsule using a microfluidic device may represent a platform for cell encapsulation treatment for T1DM and other hormone-deficient diseases.

• Authors: Zhisheng Xiao, Ting Wei, Ruiliang Ge, Qiaofeng Li, Bo Liu, Zhaoxin Ji, Linfu Chen, Junjie Zhu, Jingjing Shen, Zhuang Liu, Yueye Huang, Yang Yang, Qian Chen
• Journal: Small (Weinheim an der Bergstrasse, Germany) (Small) Vol. 18 Issue 29 Pg. e2202596 (07 2022) ISSN: 1613-6829 [Electronic] Germany
• PMID: 35733079 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 Wiley-VCH GmbH.

Chemical References

• Capsules
• Methacrylates

Topics

• Animals
• Capsules
• Diabetes Mellitus, Type 1 (therapy)
• Foreign-Body Reaction
• Islets of Langerhans Transplantation
• Methacrylates
• Mice
• Mice, Inbred C57BL
• Microfluidics
• Rats