Association between periodontitis and peripheral markers of innate immunity activation and inflammation.

Abstract

BACKGROUND:

Immune response leading to increased systemic inflammation is one of the mechanisms linking periodontitis to chronic inflammatory diseases. The aim of this study was to compare the expression of toll-like receptors 2 and 4 in monocytes and neutrophils (TLR2M, TLR2N, TLR4M, and TLR4N) and its endogenous ligands (cellular fibronectin [cFN] and heat shock protein 60 [HSP60]) in patients with and without periodontitis. Additionally, the relationship between cFN and HSP60 expression with innate immunity activation and systemic inflammatory response (interleukin 6 [IL-6]) was also evaluated.

METHODS:

A case-controlled study was designed in which 30 patients with periodontitis (cases) and 30 age- and sex-matched participants without periodontitis (controls) were included. Fasting blood samples were collected to determine: (1) expression of TLR2N, TLR2M, TLR4N, and TLR4M by flow cytometry; and (2) serum concentrations of cFN, HSP60, and IL-6 by ELISA technique.

RESULTS:

Expression of TLR2M (411.5 [314.2, 460.0] vs. 236.5 [204.0, 333.0] AFU), TLR2N (387.0 [332.0, 545.5] vs 230.0 [166.2, 277.7] AFU), TLR4M (2478.5 [1762.2, 2828.0] vs 1705.0 [1274.5, 1951.2] AFU), and TLR4N (2791.0 [2306.7, 3226.2] vs. 1866.0 [1547.5, 2687.2] AFU) as well as serum levels of cFN (301.1 [222.2, 410.9] vs. 156.4 [115.3, 194.0] ng/ml) and IL-6 (10.4 [6.5, 11.5] vs. 3.5 [2.6, 4.9] pg/ml) were significantly higher in periodontitis patients than those without periodontitis. A positive association was found between periodontitis and cFN (odds ratio [OR] = 1.028, p < 0.001), TLR2N (OR = 1.026, p < 0.001), TLR4M (OR = 1.001, p = 0.002), and IL-6 (OR = 1.774, p < 0.001).

CONCLUSIONS:

Periodontitis patients exhibited high expression of TLRs, cFN, and IL-6.

• Authors: José Dopico, João Botelho, Alberto Ouro, Clara Domínguez, Vanessa Machado, Marta Aramburu-Nuñez, Antía Custodia, Teresa Blanco, María Vázquez-Reza, Daniel Romaus-Sanjurjo, Juan Blanco, Rogelio Leira, Tomás Sobrino, Yago Leira
• Journal: Journal of periodontology (J Periodontol) Vol. 94 Issue 1 Pg. 11-19 (01 2023) ISSN: 1943-3670 [Electronic] United States
• PMID: 35665930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2022 American Academy of Periodontology.

Chemical References

• Interleukin-6

Topics

• Humans
• Interleukin-6
• Periodontitis (complications)
• Inflammation
• Immunity, Innate
• Monocytes