Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells.

Abstract

Sepsis is caused by bacterial infections or viral infections. Clinically, there exist confirmed or highly suspected infection foci. Mortality caused by septic shock remains in a high rate even though antibiotic treatment works effectively. In this study, we treat THP-1 cells with 1 ug/mL LPS (lipopolysaccharide) and add paeoniflorin or LR-12 inhibitor. TREM-1 (triggering receptor expressed on myeloid cells-1), IL-6, IL-1β, and TNF-α (tumour necrosis factor alpha (a)-cachectin) were detected by ELISA and qRT-PCR, and western blotting is performed to detect related proteins in the NF-κB signaling pathway. As a result, paeoniflorin can significantly reduce the production of LPS-stimulated TREM-1 as well as inflammatory factors and attenuate the phosphorylation of NF-κB signaling pathway-related factors, such as p65 and IκBα. At the same time, the combined effect of paeoniflorin and LR-12 is more significant. The results of this study solidly prove that paeoniflorin plays a role in inhibiting TREM-1-mediated inflammation and the NF-κB pathway could be a potential mechanism of action.

• Authors: Li Cao, Kerong Yang
• Journal: Journal of healthcare engineering (J Healthc Eng) Vol. 2022 Pg. 7051643 ( 2022) ISSN: 2040-2309 [Electronic] England
• PMID: 35480155 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Li Cao and Kerong Yang.

Chemical References

• Glucosides
• Lipopolysaccharides
• Monoterpenes
• NF-kappa B
• TREM1 protein, human
• Triggering Receptor Expressed on Myeloid Cells-1
• Tumor Necrosis Factor-alpha
• peoniflorin

Topics

• Glucosides
• Humans
• Inflammation (drug therapy)
• Lipopolysaccharides (pharmacology)
• Monoterpenes
• NF-kappa B (metabolism)
• THP-1 Cells
• Triggering Receptor Expressed on Myeloid Cells-1 (metabolism)
• Tumor Necrosis Factor-alpha (metabolism, pharmacology)