Abstract | BACKGROUND: Mesenchymal stem cells (MSCs) are promising candidates for tissue regeneration and disease treatment. However, long-term in vitro passaging leads to stemness loss of MSCs, resulting in failure of MSC therapy. This study investigated whether the combination of melatonin and human umbilical cord mesenchymal stem cells (hUC-MSCs) was superior to hUC-MSCs alone in ameliorating high-fat diet and streptozocin (STZ)-induced type II diabetes mellitus (T2DM) in a mouse model. METHODS: Mice were divided into four groups: normal control (NC) group; T2DM group; hUC-MSCs treatment alone (UCMSC) group and pretreatment of hUC-MSCs with melatonin (UCMSC/Mel) group. RESULTS: RNA sequence analysis showed that certain pathways, including the signaling pathway involved in the regulation of cell proliferation signaling pathway, were regulated by melatonin. The blood glucose levels of the mice in the UCMSC and UCMSC/Mel treatment groups were significantly reduced compared with the T2DM group without treatment (P < 0.05). Furthermore, hUC-MSCs enhance the key factor in the activation of the PI3K/Akt pathway in T2DM mouse hepatocytes. CONCLUSION:
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Authors | Aili Aierken, Balun Li, Peng Liu, Xuedi Cheng, Zheng Kou, Ning Tan, Mengfei Zhang, Shuai Yu, Qiaoyan Shen, Xiaomin Du, Bold Bayar Enkhbaatar, Juqing Zhang, Rui Zhang, Xiaolong Wu, Ruibin Wang, Xin He, Na Li, Sha Peng, Wenwen Jia, Congrong Wang, Jinlian Hua |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 13
Issue 1
Pg. 164
(04 12 2022)
ISSN: 1757-6512 [Electronic] England |
PMID | 35414044
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Phosphatidylinositols
- bcl-2-Associated X Protein
- Proto-Oncogene Proteins c-akt
- Melatonin
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Topics |
- Animals
- Diabetes Mellitus, Type 2
(therapy)
- Humans
- Insulin Resistance
- Melatonin
(pharmacology, therapeutic use)
- Mesenchymal Stem Cell Transplantation
(methods)
- Mice
- Phosphatidylinositol 3-Kinases
(metabolism)
- Phosphatidylinositols
(therapeutic use)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Signal Transduction
- Umbilical Cord
- bcl-2-Associated X Protein
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