Abstract | PURPOSE: METHODS: Plate clone formation assay was performed to detect the effect of kaempferol on cell proliferation. Flow cytometry was used to detect the impact of kaempferol on cell apoptosis and cell cycle distribution. qPCR, immunofluorescence staining, and enzyme-linked immunosorbent assay were used to detect the expression of gene and protein of Ki67 which is a biomarker of cell proliferation. RESULTS: In the present study, we found kaempferol could dramatically suppress androgen-dependent and androgen-independent prostate cancer cells proliferation and induce their apoptosis. Furthermore, we found that kaempferol induced cell cycle to be arrested at G1 phase in 22Rv1 cells but at S and G2 phase in PC-3 cells. In addition, we detected the mRNA and protein of Ki67 which is corresponding to the cell proliferation and found that kaempferol could significantly inhibit Ki67 expression at mRNA level but increase its expression at protein levels in both androgen-dependent and androgen-independent prostate cancer cells. CONCLUSION:
|
Authors | Yimin Zhang, Jingqin Chen, Wenxuan Fang, Kaoyun Liang, Xiaoning Li, Fan Zhang, Yuzhou Pang, Gang Fang, Xueni Wang |
Journal | Molecular biology reports
(Mol Biol Rep)
Vol. 49
Issue 6
Pg. 4607-4617
(Jun 2022)
ISSN: 1573-4978 [Electronic] Netherlands |
PMID | 35286519
(Publication Type: Journal Article)
|
Copyright | © 2022. The Author(s), under exclusive licence to Springer Nature B.V. |
Chemical References |
- Androgens
- Kaempferols
- Ki-67 Antigen
- RNA, Messenger
- Receptors, Androgen
|
Topics |
- Androgens
(metabolism, pharmacology)
- Apoptosis
- Cell Line, Tumor
- Cell Proliferation
- Humans
- Kaempferols
(pharmacology)
- Ki-67 Antigen
(genetics)
- Male
- Prostatic Neoplasms
(drug therapy, genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Receptors, Androgen
(genetics, metabolism)
|